南方医科大学学报2024，Vol.44Issue(1)：52-59,8.DOI:10.12122/j.issn.1673-4254.2024.01.07
高良姜素通过下调lncRNA H19的表达抑制ox-LDL诱导的人源正常主动脉内皮细胞血管生成活性
Galangin inhibits oxidized low-density lipoprotein-induced angiogenic activity in human aortic endothelial cells by downregulating lncRNA H19
摘要
Abstract
Objective To investigate the effects of galangin on angiogenic activity of oxidized low-density lipoprotein(ox-LDL)-induced human aortic endothelial cells(HAECs)and explore the underlying mechanisms.Methods HAECs incubated with 10,20,40,and 80 μmol/L galangin for 24 h were assessed for cell viability changes using MTT assay to determine the cytotoxicity of galangin.HAECs treated with 5 mg/mL ox-LDL and incubated with 20 and 40 μmol/L galangin for 24 h,and the cells overexpressing lncRNA H19 and incubated with 40 μmol/L galangin for 24 h were examined for lncRNA H19 level with qRT-PCR.The migration and tube formation capacity of the cells were observed using scratch assay and angiogenesis assay,and ROS levels in the cells were detected with flow cytometry.The protein expression levels of VEGFA,MMP-2 and MMP-9 in the treated cells were detected with Western blotting.Results Galangin at 10,20,or 40 μmol/L produced no obvious toxicity(P>0.05),whereas 80 μmol/L galangin significantly inhibited the viability of HAECs(P<0.01).Treatment with ox-LDL significantly increased the expression of lncRNA H19 in HAECs.Galangin significantly lowered lncRNA H19 expression in ox-LDL-induced HAECs,suppressed cell migration,angiogenesis and ROS production level,and reduced the protein levels of VEGFA,MMP-2 and MMP-9(P<0.01).The effects of galangin were blocked by overexpression of lncRNA H19 in the cardiomyocytes.Conclusion The therapeutic effect of galangin for atherosclerosis is mediated by inhibiting lncRNA H19 expression to reduce ox-LDL-induced migration,oxidative stress,and angiogenesis of HAECs.关键词
血管生成/人源正常主动脉内皮细胞/高良姜素/lncRNA H19Key words
angiogenesis/human aortic endothelial cells/galangin/lncRNA H19引用本文复制引用
罗瑞,田龙海,杨永曜..高良姜素通过下调lncRNA H19的表达抑制ox-LDL诱导的人源正常主动脉内皮细胞血管生成活性[J].南方医科大学学报,2024,44(1):52-59,8.基金项目
国家自然科学基金(82260094) （82260094）
贵州省科学技术基金(黔科合基础-ZK[2021]一般355) Supported by National Natural Science Foundation of China(82260094). （黔科合基础-ZK[2021]一般355）
