南方医科大学学报2024，Vol.44Issue(1)：70-82,13.DOI:10.12122/j.issn.1673-4254.2024.01.09
TTC9A在泛癌中的表达水平与多种癌症的预后和免疫微环境相关
A pan-cancer analysis of TTC9A expression level and its correlation with prognosis and immune microenvironment
摘要
Abstract
Objective To investigate the expression level of tetratricopeptide repeat protein 9A in tumors and its association with the patients'prognosis and immune infiltration.Methods TTC9A expression in different tumor tissues and its association with prognosis,DNA methylation,tumor mutation burden(TMB),and microsatellite instability(MSI)were analyzed based on data from TCGA and GTEx.TIMER and xCell were used to analyze the relationship between TTC9A expression and immune infiltration.Western blotting and RT-qPCR were used to detect the expression of TTC9A in 4 types of cancer cell lines.Results TTC9A expressions were significantly increased in many tumors and down-regulated in a few cancer types(P<0.05).Western blotting and RT-qPCR showed that TTC9A expressions were elevated in lung,colon and liver cancer cells but decreased in bladder cancer cells.In head and neck squamous cell carcinoma,renal clear cell carcinoma,renal papillary cell carcinoma,low-grade glioma,malignant mesothelioma,and endometrial carcinoma tumors,a high expression of TTC9A was strongly correlated with better overall survival(OS),disease-specific survival(DSS),and progression-free interval(PFI)(P<0.05),but was correlated with worse OS,DSS,and PFI in lung adenocarcinoma,pancreatic adenocarcinoma,adrenal carcinoma,and rectal adenocarcinoma(P<0.05).TTC9A hypermethylation was associated with a more favorable prognosis of glioblastoma multiforme,low-grade glioma,uveal melanoma,and ovarian plasmacytoid cystadenocarcinoma(P<0.05)but with poor prognosis of squamous cell carcinoma of the uterine cervix and intracervical adenocarcinoma,squamous cell carcinoma of head and neck,squamous cell carcinoma of the lungs,adrenal carcinoma,and endometrial carcinoma(P<0.05).In most of the cancer types,TTC9A was significantly correlated with the level of immune cell infiltration(P<0.05).Conclusion TTC9A can be used as a prognostic marker for a variety of cancers and is strongly associated with TBM,MSI and immune cell infiltration.关键词
TTC9A/泛癌分析/免疫微环境/预后Key words
TTC9A/pan-cancer analysis/immune microenvironment/prognosis引用本文复制引用
姚倚钠,刘佳,周想军,刘泽宇,邱士珍,何颖政,周雪琼..TTC9A在泛癌中的表达水平与多种癌症的预后和免疫微环境相关[J].南方医科大学学报,2024,44(1):70-82,13.基金项目
国家自然科学基金青年科学基金(81903350) （81903350）
广东省基础与应用基础研究基金(2021A1515010007) Supported by Natural Science Foundation for the Youth(NSFY)of China(81903350). （2021A1515010007）
