南方医科大学学报2024，Vol.44Issue(1)：100-107,8.DOI:10.12122/j.issn.1673-4254.2024.01.12
电凝法制作C57/BL6J小鼠局灶性脑缺血模型的评价
Validation of a C57/BL6J mouse model of focal cerebral ischemia established by electrocoagulation of the middle cerebral artery
摘要
Abstract
Objective To modify the method for establishing mouse models of middle cerebral artery occlusion(MCAO)-induced focal cerebral ischemia using electrocoagulation.Methods Forty-six C57/BL6J male mice were divided into MCAO model group(n=34)and sham-operated group(n=12).In the model group,MCAO was induced by permanent coagulation of the right middle cerebral artery(MCA)using a coagulator,and cerebral blood flow perfusion was monitored before and at 20 min and 1 day after modeling.Neurological deficits of the mice at 1,7,and 14 days after modeling were evaluated using Longa score,mNSS score,beam walking test,cylinder test and corner test.TTC staining was used to measure the cerebral infarct size,and Western blotting was performed to detect the expressions of BDNF,GFAP and DCX proteins in the ischemic cortex.Results The mice in the model group showed significantly reduced cerebral blood flow in the MCA on the ischemic side and obvious neurological deficits with increased forelimb use asymmetry on days 1,7 and 14 after modeling(P<0.05).In the cerebral cortex on the ischemic side of the model mice,the expressions of GFAP and DCX increased significantly at 1,7,and 14 days(P<0.05)and the expression of BDNF increased at 1 day after modeling ischemia(P<0.05).Conclusion We successfully prepared mouse models of MCAO using a modified method by changing the electrocoagulation location from the distal location of the junction between the MCA and the inferior cerebral vein to a 2 mm segment medial to the junction between the MCA and the olfactory bundle.关键词
电凝法/大脑中动脉/脑缺血小鼠/评价指标Key words
electrocoagulation/middle cerebral artery/cerebral ischemic mice/evaluation indicators引用本文复制引用
康赟赟,唐东宁,张健,夏青..电凝法制作C57/BL6J小鼠局灶性脑缺血模型的评价[J].南方医科大学学报,2024,44(1):100-107,8.基金项目
国家自然科学基金青年科学基金(82105021) Supported by National Natural Science Foundation of China Youth Science Foundation(82105021). （82105021）
