南方医科大学学报2024，Vol.44Issue(1)：156-165,10.DOI:10.12122/j.issn.1673-4254.2024.01.18
C/EBPβ介导NPHP1敲低的肾小管上皮细胞TNF-α通路下游炎症因子的表达
C/EBPβ mediates expressions of downstream inflammatory factors of the tumor necrosis factor-α signaling pathway in renal tubular epithelial cells with NPHP1 knockdown
摘要
Abstract
Objective To explore the activation of tumor necrosis factor-α(TNF-α)signaling pathway and the expressions of the associated inflammatory factors in NPHP1-defective renal tubular epithelial cells.Methods A human proximal renal tubular cell(HK2)model of lentivirus-mediated NPHP1 knockdown(NPHP1KD)was constructed,and the expressions of TNF-α,p38,and C/EBPβ and the inflammatory factors CXCL5,CCL20,IL-1β,IL-6 and MCP-1 were detected using RT-qPCR,Western blotting or enzyme-linked immunosorbent assay.A small interfering RNA(siRNA)was transfected in wild-type and NPHP1KDHK2 cells,and the changes in the expressions of TNF-α,p38,and C/EBPβ and the inflammatory factors were examined.Results NPHP1KDHK2 cells showed significantly increased mRNA expressions of TNF-α,C/EBPβ,CXCL5,IL-1β,and IL-6(P<0.05),protein expressions of phospho-p38 and C/EBPβ(P<0.05),and IL-6 level in the culture supernatant(P<0.05),and these changes were significantly blocked by transfection of cells with siRNA-C/EBPβ(P<0.05).Conclusion TNF-α signaling pathway is activated and its associated inflammatory factors are upregulated in NPHP1KDHK2 cells,and C/EBPβ may serve as a key transcription factor to mediate these changes.关键词
肾单位肾痨/TNF-α/C/EBPβ/炎症因子/NPHP1Key words
nephronophthisis/tumor necrosis factor-α/C/EBPβ/inflammatory factor/NPHP1引用本文复制引用
黄丹梅,刘雅清,李丹彤,张静兰,杨翌晨,孙良忠..C/EBPβ介导NPHP1敲低的肾小管上皮细胞TNF-α通路下游炎症因子的表达[J].南方医科大学学报,2024,44(1):156-165,10.基金项目
广东省自然科学基金(2022A1515012307 （）
2020A1515010286) （）
北京白求恩公益基金会(SCE093DS) （SCE093DS）
南方医科大学南方医院院长基金青年项目(2020C021) （2020C021）
