桑白皮提取物抑制高迁移率族蛋白/Toll样受体4通路对肺炎支原体肺炎大鼠的保护机制OACSTPCD
Protective Mechanism of Cortex Mori Extract in Inhibiting High Mobility Group Protein/Toll-Like Receptor 4 Pathway in Rats with Mycoplasma Pneumoniae Pneumonia
目的:探究桑白皮提取物对肺炎支原体(MP)肺炎大鼠的药效,以及对高迁移率族蛋白(HMGB-1)/Toll样受体4(TLR4)信号通路的影响.方法:将Wistar雄性大鼠随机分为对照组、MP肺炎模型组、阿奇霉素(阳性对照)组及桑白皮提取物低、中、高剂量组[2、4、8 g/(kg·d)],每组各12只.除对照组外,其余大鼠构建MP肺炎大鼠模型,并给予相应剂量的药物处理.显微镜下计数肺泡灌洗液(BALF)中炎性细胞计数;采用酶联免疫吸附试验(ELISA)法检测血清中炎性细胞因子水平;苏木精-伊红(HE)染色观察肺组织病理变化;Western blotting法和免疫组化检测肺组织HMGB-1、TLR4、p-NF-κB p65蛋白表达水平.结果:对照组大鼠肺组织细胞排列正常,无病变;MP肺炎模型组大鼠肺组织异常,大量炎性细胞浸润;经桑白皮提取物给药后,大鼠肺组织损伤明显减轻,炎性细胞浸润明显减少.与对照组相比,MP肺炎模型组大鼠肺系数、BALF中炎性细胞计数、血清中白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α水平及肺组织中HMGB-1、TLR4、p-NF-κB p65蛋白水平升高,IL-10水平降低(P＜0.05).与模型组相比,桑白皮提取物各剂量组和阿奇霉素组大鼠上述指标趋势相反(P＜0.05).结论:桑白皮提取物可能通过抑制HMGB-1/TLR4信号通路,减轻炎症反应,发挥对MP肺炎大鼠的保护作用.
Objective:To explore the efficacy of cortex mori extract in the treatment of rats with Mycoplasma pneumonia(MP)pneumonia and its effects on high mobility group protein(HMGB-1)/Toll-like receptor 4(TLR4)signaling pathway.Methods:Wistar male rats were randomly divided into the control group,MP pneumonia model group,azithromycin group(positive control),and low,medium and high dose cortex mori extract groups(2,4 and 8 g/(kg·d)),with 12 rats in each group.Except for the control group,other rats were used to establish the MP pneumonia rat model and given the corresponding doses of drugs.The total number of inflammatory cells in the bronchoalveolar lavage fluid(BALF)was counted under the microscope,the levels of inflammatory cytokines in serum were detected by enzyme-linked immunosorbent assay(ELISA),the pathological changes of lung tissue were observed by Hematoxylin-Eosin(HE)staining,the expression levels of HMGB-1,TLR4,and p-NF-κB p65 proteins in lung tissues were determined by Western blotting and immunohistochemistry.Results:The pulmonary tissue cells of rats in the control group were arranged normally without any lesions,the pulmonary tissue of rats in the MP pneumonia model group displayed abnormalities with a large number of inflammatory cells infiltration.After the administration of cortex mori extract,the pulmonary tissue damage of rats decreased significantly,and the inflammatory cell infiltration decreased markedly.Compared with the control group,the MP pneumonia model group exhibited a significant increases in the pulmonary coefficient,total number of inflammatory cells in BALF,serum levels of interleukin(IL)-6 and tumor necrosis factor(TNF)-α,as well as the protein levels of HMGB-1,TLR4 and p-NF-κB p65 in pulmonary tissue,along with a significant decrease in IL-10 levels(P＜0.05).Compared with the model group,the above mentioned indicators of rats in each dose group of cortex mori extract and azithromycin group showed reverse trends(P＜0.05).Conclusion:Cortex mori extract may reduce inflammation by inhibiting HMGB-1/TLR4 signaling pathway,and play a protective role in rats with MP pneumonia.
夏家敏;马娟萍;张蓉;于英
甘肃省酒泉市人民医院,甘肃酒泉 735000
药学
桑白皮提取物肺炎支原体炎症反应高迁移率族蛋白Toll样受体4
cortex mori extractMycoplasma pneumoniaeinflammatory responsehigh mobility group proteinToll-like receptor 4
《儿科药学杂志》 2024 (001)
1-5 / 5
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