福建农业科技2023，Vol.54Issue(9)：32-41,10.DOI:10.13651/j.cnki.fjnykj.2023.09.005
KASP技术在动物资源鉴定及标记辅助育种中的应用
Application of KASP Technology in Animal Resource Identification and Marker-assisted Breeding
摘要
Abstract
Kompetitive allele-specific PCR(KASP)was a fluorescence-based homogeneous genotyping technique,which could perform the biallelic genotyping detection on the single nucleotide polymorphism sites and insertion/dele-tion polymorphisms in the genome.It had the advantages of rapid detection,low cost and high accuracy.The compo-sition,amplification principle and reaction steps of KASP detection system were described in this paper,as well as its advantages over the traditional SNP detection methods.Then,the current research progresses in animal genetic iden-tification,functional gene analysis and molecular marker-assisted selection(MAS)assisted breeding were summa-rized,in order to provide reference for the application of KASP technology in animal field.关键词
KASP/SNP/遗传鉴定/分子标记辅助育种Key words
KASP/SNP/Genetic identification/Molecular marker-assisted breeding分类
生物学引用本文复制引用
符宏高,杨理程,吴锐琼,黄镇..KASP技术在动物资源鉴定及标记辅助育种中的应用[J].福建农业科技,2023,54(9):32-41,10.基金项目
福建省自然科学杰出青年基金项目(2023J06027). （2023J06027）
