福建农业科技2023，Vol.54Issue(9)：42-46,5.DOI:10.13651/j.cnki.fjnykj.2023.09.006
Cd胁迫下水稻Cd亚细胞特征及游离氨基酸含量变化分析
Analysis of Cd Subcellular Characteristics and the Changes of Free Amino Acid Contents in Rice Under Cd Stress
摘要
Abstract
By taking the rice varieties of Nipponbare and Lemont as the materials,the effects of Cd stress on the con-tent of pigment,the subcellular distribution of Cd and the content of free amino acid in different rice varieties were ex-plored by being treated with adding different concentrations of Cd in the hydroponic nutrient solution.The results showed that with the increase of Cd concentration,the two kinds of rice showed a decreasing trend of the contents of chlorophyll and carotenoid,while the content of Cd in cell wall,cytoplasm and organelle increased significantly.The Cd content in cell wall of the rice varieties of Nipponbare and Lemont under different treatments accounted for 86.24％-88.59％and 88.66％-94.17％of the total contents of Cd in cells,respectively,indicating that the cell wall was the main part of Cd accumulation in rice.When treated with Cd of 50 μmol·L-1 and Cd of 100 μmol·L-1,the contents of proline,cysteine and glutamic acid in the two rice varieties of Nipponbare and Lemont increased signif-icantly with the increase of Cd concentration.Under the treatment of 50 μmol·L-1 Cd,the contents of alanine,phenylalanine,methionine and glycine in the two rice varieties could be increased significantly.However,under the treatment of 100 μmol·L-1 Cd,However,the contents of phenylalanine and methionine in Nipponbare rice and ala-nine,methionine and glycine in Lemont rice were not significantly increased.In summary,under the stress of Cd,the two rice varieties enhanced the resistance of Cd by adjusting the subcellular distribution of Cd and accumulating the content of free amino acids,and showed the genotypic differences between Nipponbare and Lemont varieties.关键词
水稻/Cd胁迫/亚细胞分布/游离氨基酸Key words
rice/Cd stress/subcellular distribution/free amino acid分类
资源环境引用本文复制引用
林红梅,李齐向..Cd胁迫下水稻Cd亚细胞特征及游离氨基酸含量变化分析[J].福建农业科技,2023,54(9):42-46,5.基金项目
国家自然科学基金青年项目(32001109) （32001109）
福建省自然科学基金面上项目(2023J011577) （2023J011577）
闽江学院校级项目(MJUJG202213034). （MJUJG202213034）
