福建农业科技2023，Vol.54Issue(9)：69-74,6.DOI:10.13651/j.cnki.fjnykj.2023.09.011
大肠杆菌过表达EutJ、EutG对庆大霉素的耐药性分析
Drug Resistance Analysis of the Overexpression of EutJ and EutG to Gentamicin
摘要
Abstract
In order to screen the new drug-resistant key genes and study their drug resistance mechanisms to alleviate the problem of bacterial resistance,by constructing the overexpression strains of the ethanolamine-utilizing chaper-one protein EutJ and the alcohol dehydrogenase EutG,the killing tests of gentamicin were performed on the two strains.Then,by taking the killing effect of the wild-type Escherichia coli carrying the empty plasmid in logarithmic phase as the control group,the minimum inhibitory concentration(MIC)was further determined,and the correlation between the ethanolamine metabolism and bacterial tolerance was studied through the detection of antibiotic uptake by inhibition zone.The results showed that after the overexpression of EutJ and EutG,Escherichia coli could reduce the concentrations of antibiotics in the bacteria to improve the survival rate under the condition of gentamicin killing,which proved that the genes of eutJ and eutG could be used as the potential tolerance-related genes to help the bacteria escape from the antibiotic attacks.Therefore,the key enzymes of ethanolamine metabolism,EutJ and EutG,were expected to be the targets for the development of immune adjuvants or new antibiotics.关键词
乙醇胺利用伴侣蛋白EutJ/醇脱氢酶EutG/庆大霉素/耐药性Key words
Ethanolamine-utilizing chaperone protein EutJ/Alcohol dehydrogenase EutG/Gentamicin/Drug resistance分类
生物科学引用本文复制引用
翁嘉仪,俞丹丹,张裕丹,高媛媛..大肠杆菌过表达EutJ、EutG对庆大霉素的耐药性分析[J].福建农业科技,2023,54(9):69-74,6.基金项目
福建省自然科学基金面上项目(2020J01175). （2020J01175）
