福建农业学报2023，Vol.38Issue(10)：1133-1138,6.DOI:10.19303/j.issn.1008-0384.2023.10.001
水稻肽转运蛋白基因OsPtr1的功能分析
Functions of OsPtr1 in Rice
摘要
Abstract
[Objective]Functions of a peptide transporter protein gene,OsPtr1,in rice were studied by means of T-DNA insertion mutation.[Method]The target RNA was extracted from the endosperm of Minghui 86 rice 12 d after flowering and reverse-transcribed into cDNA.By cloning the candidate gene P0421H01.23,named OsPtr1,and constructing the plant overexpression vector using the promoter Gt1 of endosperm-specific expressing,OsPtr1 was introduced into Nipponbare rice using an agrobacterium-mediated method.Nitrogen metabolism of the offspring and mutant w9101 was analyzed.A fusion expression vector of green fluorescent protein gene,GFP,and OsPtr1 was constructed and transferred to onion epidermal cells with a gene gun.In 24-48 h afterward,subcellular localization of OsPtr1 was examined under a fluorescence confocal electron microscope.[Result]OsPtr1 was localized at the membrane and possibly involved in the transport of nitrogen substances.Knockout on OsPtr1 could cause a reduction on nitrogen-containing substances,such as amino acids and proteins,in the rice grains.Conversely,an overexpressed OsPtr1 would increase the accumulation.[Conclusion]OsPtr1 participated in the transmembrane transport of nitrogen substances in rice.关键词
水稻/胚乳特异表达/肽转运蛋白/OsPtr1/氮代谢Key words
Rice/endosperm-specific expressing/peptide transporter/OsPtr1/nitrogen-metabolism分类
农业科技引用本文复制引用
胡昌泉,刘华清,李刚,王锋..水稻肽转运蛋白基因OsPtr1的功能分析[J].福建农业学报,2023,38(10):1133-1138,6.基金项目
福建省科技计划公益类专项(2021R10270010) （2021R10270010）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021002) （XTCXGC2021002）
