福建农业学报2023，Vol.38Issue(10)：1139-1145,7.DOI:10.19303/j.issn.1008-0384.2023.10.002
盐胁迫对藜麦种子萌发生长的影响及耐盐机理研究
Growth and Saline-tolerance of Quinoa Seeds and Seedlings under Salt Stress
摘要
Abstract
[Objective]Germination,growth,and physiology of quinoa seeds and seedlings under salt stress were analyzed.[Method]Temuco Quinoa seeds and potted seedlings were treated with NaCl solutions of different concentrations(i.e.,0,200,and 450 mmol·L-1).Physiological indexes on seed germination and seedling growth,agronomic traits of mature plants,contents of nutrients in the seeds or seedlings,and spatial-temporal expression of SOD,POD,and BADH in the seedlings,were monitored to determine the effects of the imposed salt stress.[Result](1)Quinoa seed germination and seedling growth were significantly inhibited by the high NaCl concentration at 450 mmol·L-1.The N-uptake was ill-affected,the Na+/K+ ratio significantly increased,the activities of superoxide dismutase(SOD)and peroxidase(POD)significantly decreased,while malondialdehyde(MDA)content significantly higher than that of control.However,under 200 mmol NaCl·L-1,the quinoa plants grew and developed well.Aside from a declined germination index,the seed vigor index and fresh seedling weight were significantly increased,the content of all nutrients slightly raised,and the SOD and POD activities significantly elevated without a significant difference in MDA over control.(2)Under the salt stress of 200 mmol·L-1 and 450 mmol·L-1,the soluble sugar content increased by 68.06%and 41.67%,and the proline by 237.38%and 189.97%,respectively.As the salt concentration increased,they share a similar trend of firstly increasing then a decline.(3)In response to salt stress,BADH and SOD were more highly expressed in the root tissues than in the stems and leaves,while POD was highest in the leaves and lowest in the roots.[Conclusion]High salt concentration at 450 mmol·L-1 ill-affected the nutrient content in Temuco quinoa seeds,inhibited the germination,and hindered the seedling development.The temporal expression of BADH was more rapid and sensitive to salt stress than those of POD and SOD at either 200 mmol·L-1 or 450 mmol·L-1.Consequently,it could be served as an indicator in screening salt-tolerant quinoa germplasms.关键词
藜麦/生理生化/抗逆基因/耐盐机理Key words
Quinoa/physiology/resilience genes/salt tolerance mechanism分类
农业科技引用本文复制引用
邓焕莹,钱峰,周琬敏,陈雨寒,颜统利,赵燕昊,蒋玉蓉,陆国权..盐胁迫对藜麦种子萌发生长的影响及耐盐机理研究[J].福建农业学报,2023,38(10):1139-1145,7.基金项目
浙江省自然科学基金项目(Y21C130006) （Y21C130006）
浙江省重点研发项目(2021C02057) （2021C02057）
浙江省农业新品种选育重大科技专项子课题(2016C02050-9-9) （2016C02050-9-9）
浙江农林大学学生科研训练项目(202210341235) （202210341235）
