福建农业学报2023，Vol.38Issue(11)：1259-1266,8.DOI:10.19303/j.issn.1008-0384.2023.11.001
玉米咖啡酸O-甲基转移酶的全基因组鉴定与功能分析
Identification and Functions of Caffeic Acid O-methyltransferase Genes in Maize
摘要
Abstract
[Objective]Localization in genome and response to salt stress of the genes of caffeic acid O-methyltransferase(COMT),a key melatonin biosynthesis enzyme relating to plant growth,development,and stress resistance,in maize were studied.[Method]Structure,phylogenetics,and expressions of the COMT family in maize(ZmCOMT)were analyzed.Functions of the genes were verified by overexpressing ZmCOMT12 in Arabidopsis thaliana.[Result]Twenty-eight COMTs were identified in the maize genome and phylogenetically divided into Branch Ⅰand Branch II.Most of them were structurally similar with a like motif composition,and some had tissue specific expressions.As revealed by RT-PCR,ZmCOMTs could be induced by salt stress,indicating its association with the stress regulation.The overexpressed ZmCOMT12 significantly elevated the melatonin content and salt tolerance of Arabidopsis.[Conclusion]ZmCOMT12 was hypothesized as the melatonin synthesizing gene by the sequence alignment,genetic relationship,and protein structure analyses.关键词
玉米/咖啡酸O-甲基转移酶/褪黑素/非生物胁迫/成员鉴定Key words
Maize/caffeic acid O-methyltransferase/melatonin/abiotic stress/member identification分类
农业科技引用本文复制引用
张璐,赵月强,付家锋,张权,马春业,李英壮,张兰,王改革..玉米咖啡酸O-甲基转移酶的全基因组鉴定与功能分析[J].福建农业学报,2023,38(11):1259-1266,8.基金项目
国家玉米产业技术体系建设专项(nycytx-02) （nycytx-02）
