福建农业学报2023，Vol.38Issue(11)：1293-1301,9.DOI:10.19303/j.issn.1008-0384.2023.11.005
文心兰花粉离体萌发培养基的筛选及萌发率的测定
Optimized Medium and Culture Conditions for Germination of Oncidium Pollens
摘要
Abstract
[Objective]Medium formulation and culture conditions were optimized for in vitro pollen germination of Oncidium spp.[Methods]On a liquid culture medium,in vitro germination rates of the pollens collected from the flowers of various Oncidium germplasms on the day of full bloom were determined.Based on the effects of sucrose,H3BO3,Ca(NO3)·4H2O,MgSO4·7H2O,and KNO3 on the germination rate,an orthogonal experiment was applied to optimize the medium formulation and culture conditions.Subsequently,on the optimized medium under selected conditions,in vitro pollen germination rates of 68 collected Oncidium germplasms were determined.[Results]The degrees of importance of the medium ingredients to the pollen germination were found to be H3BO3＞Ca(NO3)·4H2O＞sucrose＞MgSO4·7H2O＞KNO3.The optimized formulation consisted of 100 g·L-1 of sucrose,10 mg·L-1 of H3BO3,50 mg·L-1 of Ca(NO3)·4H2O,20 mg·L-1 of MgSO4·7H2O,and 30 mg·L-1 of KNO3.The pollen culture was conducted at 25℃for 48h in darkness to deliver a generally low germination rate of 0.74%to 49.65%on 60.29%of the Oncidium germplasms.[Conclusion]The liquid medium and culture conditions for an in vitro pollen germination of Oncidium were optimized.The germination rates differed significantly among the species.关键词
文心兰/花粉/离体萌发/液体培养基/萌发率Key words
Oncidium spp./pollen/in vitro germination/liquid medium/germination rate分类
农业科技引用本文复制引用
罗远华,陈燕,方能炎,林榕燕,钟淮钦,黄敏玲..文心兰花粉离体萌发培养基的筛选及萌发率的测定[J].福建农业学报,2023,38(11):1293-1301,9.基金项目
福建省农业科学院对外合作项目(DWHZ-2022-09) （DWHZ-2022-09）
福建省农业科学院科技创新团队建设项目(CXTD2021010-2) （CXTD2021010-2）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021016) （XTCXGC2021016）
