福建农业学报2023，Vol.38Issue(11)：1329-1336,8.DOI:10.19303/j.issn.1008-0384.2023.11.009
老鼠簕真菌病病原的分离鉴定及生物学特性
Isolation,Identification,and Biological Characteristics of a Fungal Pathogen on Acanthus ilicifolius
摘要
Abstract
[Objective]Pathogen of the leaf spot disease on Acanthus ilicifolius was isolated and identified with its biological characteristics determined.[Method]Microbes were isolated from the diseased A.ilicifolius leaves collected from the Zhangjiangkou Estuary Mangrove National Nature Reserve in Fujian Province by the detached tissue method.Morphological and molecular biological techniques including ITS and EF1-α gene sequence analysis were employed to identify the pathogen.Optimal growth conditions of the identified fungus with respect to temperatures,pH,carbon sources,and nitrogen sources,in addition to its activities of catalase(CAT),superoxide dismutase(SOD),peroxidase(POD),laccase,cellulase(CL),and β-glucosidase(β-GC)were determined.[Result]For the first time Fusarium verticillioides in the Ascomycota phylum was identified as the responsible pathogen of the disease.It thrived at 25°C and pH 10 on a medium using starch as carbon source and yeast powder as nitrogen source.POD was the oxidoreductase,and CL the hydrolase,that showed the highest activity in the fungus.[Conclusion]The pathogen that caused the leaf spot disease on bougainvillea in the Zhangjiang Estuary was identified as F.verticillioides.The biological characteristics of the strongly adaptable fungus were determined.关键词
老鼠簕/叶斑病/鉴定/轮枝镰刀菌/生物学特性Key words
Acanthus ilicifolius/leaf spot disease/identification/Fusarium verticillium/biological characteristics分类
农业科技引用本文复制引用
马林,袁宗胜,郑瑜婷,郑雄,陈维峰,王思凡,谢宝贵,刘芳..老鼠簕真菌病病原的分离鉴定及生物学特性[J].福建农业学报,2023,38(11):1329-1336,8.基金项目
国家自然科学基金项目(32002108) （32002108）
闽江学院引进人才预研项目(33004328) （33004328）
