干旱地区农业研究2024，Vol.42Issue(1)：152-158,7.DOI:10.7606/j.issn.1000-7601.2024.01.15
芝麻苗期干旱生理生化指标响应特征及其基因表达分析
Response characteristics of physiological and biochemical indices and gene expression analysis of sesame during seedling stage under drought
摘要
Abstract
To establish a drought resistance identification system for sesame and screen drought resistant varie-ties,a pot repeated drought method was used,with normal water content(CK)and drought stress(DS)treatments set up.31 sesame materials were subjected to super physiological and biochemical indicators determination and comprehensive evaluation,and the expression level of SOD synthesis related genes was detected using qRT-PCR.Results of the analysis showed that O2·-,SS,Pro contents,SOD,and POD activities under drought stress were ob-viously increased compared with the control group.The highest coefficient of variation of drought resistance coeffi-cient and drought resistance index were SOD(98.64％)and O2·-(154.01％).The drought resistance index was sig-nificantly positively correlated with O2·- content and SOD activity,which could be used as important indicators for drought resistance identification of sesame seedlings.31 sesame materials were divided into 5 categories by cluste-ring analysis:high drought-resistance,medium drought-resistance,low drought-resistance,low drought-sensitive and high drought-sensitive.Using a comprehensive evaluation method,2 high drought resistance materials('Fenzhi 10'and'Yu-11-12'),4 medium drought-resistance materials,9 low drought-resistance materials,10 drought-sensitivity materials,and 5 highly drought-sensitivity materials were selected.The content of O2·-and the activity of SOD can be used as the indexes for rapid identification of drought resistance of sesame.关键词
芝麻/抗旱生理指标/抗旱性评价/SOD合成相关基因表达Key words
Sesamum indicuim L./physiological indicators of drought resistance/drought resistance identifica-tion/SOD synthesis related gene expression分类
农业科技引用本文复制引用
刘文萍,刘霞霞,韩俊梅,文飞,吕伟,王若鹏..芝麻苗期干旱生理生化指标响应特征及其基因表达分析[J].干旱地区农业研究,2024,42(1):152-158,7.基金项目
国家特色油料产业技术体系项目(CARS-14-2-02) （CARS-14-2-02）
山西农业大学生物育种工程项目(YZGC048) （YZGC048）
吕梁市重点研发项目(2022NYGG19) （2022NYGG19）
校科技创新提升工程项目(CXGC2023067) （CXGC2023067）
