干旱地区农业研究2024，Vol.42Issue(1)：237-241,251,6.DOI:10.7606/j.issn.1000-7601.2024.01.24
附球属真菌DT-014对杂草的致病性及生物学特性研究
Study on herbicidal activity and biological characteristics of Epicoccum strain DT-014
摘要
Abstract
Fungus DT-014 of the Epicoccum,which was isolated from the leaves of Cephalanoplos setosum(Witld.)with naturally occurring disease in Yaozigou,Datong County,Xining City,Qinghai Province,was used as the research object.The pathogenicity of the strain DT-014 to 4 potted weeds Galium spurium L.,Chenopodium album L.,Malva verticillate L.var.Crispa,Elsholtzia densa Benth.and the safety of 5 potted crops Vicia faba L.,Pisum sativum L.,Hordeum vulgare L.,Triticum aestivum L.,Brassica napus L.were determined by fermentation spray inoculation.The single factor experiment method was used to explore the optimal growth medium,carbon source,nitrogen source and solid-state fermentation substrate of strain DT-014 by measuring the colony diameter and the OD600 value of spore suspension.The experimental results showed that DT-014 had strong pathogenicity to G.spurium and C.album,followed by E.densa and low pathogenicity to M.crispa.DT-014 was relatively safe for wheat T.aestivum,broad bean P.sativum,highland barley H.vulgare,and pea P.sativum,and slightly affected B.napus.The optimum medium for DT-014 was peptone medium,the optimum carbon source was glucose,the op-timum nitrogen source was yeast extract,and the optimum solid-state fermentation substrate was soybean powder.关键词
微生物除草剂/附球属真菌/杂草致病性/作物安全性/最适培养基Key words
microbial herbicides/Epicoccum/pathogenicity of weeds/crop safety/optimal culture medium分类
农业科技引用本文复制引用
朱海霞..附球属真菌DT-014对杂草的致病性及生物学特性研究[J].干旱地区农业研究,2024,42(1):237-241,251,6.基金项目
青海省科技厅国际合作专项(2023-HZ-808) （2023-HZ-808）
