桂林理工大学学报2023，Vol.43Issue(4)：679-684,6.DOI:10.3969/j.issn.1674-9057.2023.04.018
黄孢原毛平革菌胞外多糖的结构及自组装行为
Structural characterization and self-assembly behavior of extracellular polysaccharides from Phanerochaete chrysosporium
摘要
Abstract
The purpose of this study is to analyze the structure and self-assembly behavior of extracellular poly-saccharides produced by Phanerochaete chrysosporium BKMF-1767.The structure of extracellular polysaccha-rides from Phanerochaete chrysosporium is analyzed with Congo red test,molecular weight determination,X-ray diffraction and Fourier transform infrared spectroscopy,and the self-assembly behavior of the polysaccharides is studied with fluorescence microscopy,scanning electron microscopy and transmission electron microscopy.In Congo red test,the maximum absorption wavelength of the polysaccharide-Congo red solution shows obvious red-shift phenomenon in low-concentration alkali solution,and the red-shift phenomenon has disappeared with the increase of alkali solution concentration,indicating that the extracellular polysaccharides from Phanerochaete chrysosporium has a triple helix structure and the structure is destroyed into disordered structure in high concen-tration alkali solution.During molecular weight determination,the molecular weight of the polysaccharide in dimethyl sulfoxide solution is significantly lower than that in aqueous solution,indicating that the configuration of the polysaccharide chain is depolymerized in dimethyl sulfoxide solution.Fourier transform infrared spectros-copy analysis finds that the polysaccharide has characteristic absorption peaks of polysaccharide compounds and contained β-glycosidic bonds.X-ray diffraction analysis shows that it is a kind of amorphous polysaccharide.The comprehensive analysis of fluorescence microscopy,scanning electron microscopy and transmission electron microscopy indicates that the polysaccharide can form a fibrous network-like spatial structure and form aggre-gates under its self-assembly behavior.The above results indicate that the extracellular polysaccharides from Phanerochaete chrysosporium is one kind of amorphous polysaccharide with triple helical structure,which can self-assemble into fibrous aggregates.关键词
黄孢原毛平革菌/胞外多糖/三螺旋结构/链构象/自组装Key words
Phanerochaete chrysosporium/extracellular polysaccharides/triple helix structure/chain conforma-tion/self-assembly分类
生物科学引用本文复制引用
李宁杰,陈刘,刘洁,杨锐,王小洁..黄孢原毛平革菌胞外多糖的结构及自组装行为[J].桂林理工大学学报,2023,43(4):679-684,6.基金项目
国家自然科学基金项目(51608142) （51608142）
广西科技计划项目(2018GXNSFGA281001 （）
桂科AD19110151 （）
2016GXNSFBA380076) （）
