国际老年医学杂志2024，Vol.45Issue(1)：46-52,7.DOI:10.3969/j.issn.1674-7593.2024.01.009
YTHDF1对缺氧复氧损伤后H9c2心肌细胞凋亡及氧化应激的影响
Effect of YTHDF1 on Apoptosis and Oxidative Stress in Myocardial H9c2 Cells with Anoxia/Reoxygenation Injury
摘要
Abstract
Objective To investigate the effect of YTH domain family protein 1(YTHDF1)on apoptosis and oxidative damage in H9c2 cells under hypoxic reoxygenation(H/R)conditions.Methods The H/R model was constructed with H9c2 cells.Cells were transfected with YTHDF1 small interfering RNA(siRNA)and overexpression plasmid,and cell viability was measured by MTT colorimetric assay.Apoptosis,superoxide dismutase(SOD)activity,malondialdehyde(MDA)content in cells and lactate de-hydrogenase(LDH)activity in cell culture medium supernatant were measured by Hirst staining and flow cytometry.The protein lev-els of YTHDF1,cleaved caspase-3,Bax and FasL in the cells were determined by Western blot,and the apoptosis and oxidative damage of H9c2 cells in the control,H/R,YTHDF1 gene interference and overexpression groups were compared.Results LDH ac-tivity and MDA content were significantly increased,SOD activity and cell viability were significantly decreased after H/R injury(P＜0.05),and the protein levels of YTHDF1 in H9c2 cells were significantly increased after H/R treatment(P＜0.05),YTHDF1 interference alleviated the morphological changes caused by H/R injury,and YTHDF1 overexpression increased the morphological changes caused by H/R injury;cleaved caspase-3,Bax and FasL protein expression was significantly higher after H/R injury treat-ment than in the control group(P＜0.05),cleaved caspase-3,Bax and FasL protein levels were significantly lower in the H/R+pcDNA3.1-YTHDF1 group than in the H/R group(P＜0.05)and cleaved caspase-3,Bax and FasL protein levels were signifi-cantly higher in the H/R+pcDNA3.1-YTHDF1 group than in the H/R group(P＜0.05).Conclusion Downregulation of YTH-DF1 inhibited the up-regulation of cleaved caspase-3,Bax and FasL protein levels and decreased oxidative damage in H/R-trea-ted H9c2 cells.关键词
YTH域家族蛋白1/缺氧复氧/H9c2细胞/凋亡/氧化应激Key words
YTH domain family protein 1/Hypoxia-reoxygenation/H9c2 cells/Apoptosis/Oxidative stress引用本文复制引用
涅鲁排尔·热依木江,金颖,罗荔,张雅玲,刘刚,李雪,罗梅..YTHDF1对缺氧复氧损伤后H9c2心肌细胞凋亡及氧化应激的影响[J].国际老年医学杂志,2024,45(1):46-52,7.基金项目
新疆维吾尔自治区自然科学基金项目(2022D01C323) （2022D01C323）
