高校化学工程学报2023，Vol.37Issue(6)：943-950,8.DOI:10.3969/j.issn.1003-9015.2023.06.009
基于特异性引物高效构建TALE文库
Efficient construction of TALE library based on specific primers
摘要
Abstract
Artificially constructed transcriptional activator like effectors(TALE)are widely used in gene transcription regulation and genome editing.A new method for constructing a TALE library was invented due to the complexity of TALE construction processes.Based on the golden gate restriction enzyme digestion method,three backbone plasmids of TALE were first designed to recognize the guanine(G).Three backbone plasmids were then amplified with four specific primers to obtain plasmid mutants.The final TALE library was successfully constructed by connecting three plasmid mutants with the golden gate restriction enzyme digestion method.The results of gene sequencing show the library contains all nucleotides that could determine the specificity of TALE-DNA binding.This provides the best material for researchers to use TALE directly.The TALE library can be widely used in life sciences for its high targeting and versatility.关键词
TALE文库/基因转录调控/基因组编辑/特异性引物/金门酶切连接方法Key words
TALE library/gene transcription regulation/genome editing/specific primers/golden gate restriction enzyme digestion method分类
生物科学引用本文复制引用
蔡思杰,张书衍,王晓微,郭显阳,詹硕..基于特异性引物高效构建TALE文库[J].高校化学工程学报,2023,37(6):943-950,8.基金项目
国家级大学生创新创业训练计划平台-创新训练项目(202211035010X). （202211035010X）
