甘蔗糖业2023，Vol.52Issue(6)：7-11,5.
云南石屏蔗区甘蔗白条病菌的分子鉴定
Molecular Identification of the Pathogen Causing Sugarcane Leaf Scald Disease in Shiping,Yunnan Province of China
摘要
Abstract
To identify the pathogen of suspected sugarcane leaf scald plants observed in Shiping,Yunnan Province in 2023,the specific primers XAF1/XAR1 of Xanthomonas albilineans(Ashby)Dowson was used to perform PCR molecular identification for 32 suspected samples.The results showed that all samples were amplified a specific band with a size of about 578 bp,and GenBank accession number is OR495793-OR495802.The homology and phylogenetic analysis of the obtained sequences showed that it was 99.8%～100%identical to the sequence of the adenosine triphosphate-biding cassette transporter gene abc(GenBank accession number:MH709206)in X.albilineans strain Xa-FJ1.Then these X.albilineans strains sequences were clustered together into one branch in the phylogenetic tree.Base on the result of symptom diagnosis in the field and molecular identification,the sugarcane disease occurred in Shiping was confirmed to be leaf scald caused by X.albilineans.This is the first occurrence of sugarcane leaf scald disease in Shiping area of Yunnan Province.These results provide a scientific basis for the effective control of sugarcane leaf scald disease in the future.关键词
云南石屏/甘蔗白条病/病原分子鉴定Key words
Shiping in Yunnan Province/Sugarcane leaf scald disease/Pathogen molecular detection分类
农业科技引用本文复制引用
王晓燕,李婕,李文凤,张荣跃,王长秘,单红丽,黄应昆..云南石屏蔗区甘蔗白条病菌的分子鉴定[J].甘蔗糖业,2023,52(6):7-11,5.基金项目
财政部和农业农村部国家现代农业产业技术体系专项资金资助(CARS-170303) （CARS-170303）
云岭产业技术领军人才培养项目"甘蔗有害生物防控"(2018LJRC56) （2018LJRC56）
云南省现代农业产业技术体系建设专项资金 （）
