葛根异黄酮抑制XOD和GLUT9分别发挥减少尿酸生成、促进尿酸排泄双重途径的机制OACSTPCD
Pueraria isoflavones inhibit XOD and GLUT9 to decrease uric acid production and promote uric acid excretion,respectively
目的:分析葛根异黄酮抑制XOD和GLUT9分别发挥减少尿酸生成、促进尿酸排泄的可能机制.方法:2021年8月～2022年4月,采用随机数字表法将40只SPF级雄性昆明小鼠分为健康组(1次d频率250 mg/kg剂量灌胃羧甲基纤维素钠)、模型组(HUA小鼠1次d频率250 mg/kg剂量灌胃羧甲基纤维素钠)和低(HUA小鼠1次d频率125 mg/kg剂量灌胃葛根异黄酮)、中(HUA小鼠1次d频率250 mg/kg剂量灌胃葛根异黄酮)、高(HUA小鼠1次d频率500 mg/kg剂量灌胃葛根异黄酮)剂量组,每组各8只.比较各组干预前和干预后(30 d)血清和尿液中尿酸(SUA)、尿素氮(BUN)、肌酐(SCr)含量的统计学差异,比较各组干预后(30 d)肾脏组织中黄嘌呤氧化酶(XOD)和人葡萄糖转运蛋白9(GLUT9)、环氧化酶-2(COX-2)、肿瘤坏死因子(TNF-ɑ)、白细胞介素-1(IL-1β)含量的统计学差异.结果:干预后比较,模型组肾脏炎症因子(COX-2、TNF-ɑ、IL-1β);血液和尿液 3项指标(SUA、BUN、SCr);XOD、GLUT9含量均高于健康组(P<0.05);低、中、高剂量组肾脏炎症因子(COX-2、TNF-ɑ、IL-1β);血液和尿液 3项指标(SUA、BUN、SCr);XOD、GLUT9含量均低于模型组,且有低>中>高剂量组,两两分组的比较均有统计学意义(P<0.05).干预后相比干预前,血液或尿液3项指标(COX-2、TNF-ɑ、IL-1β)含量均有下降,组内比较的差异均有统计学意义(P<0.05).结论:葛根异黄酮能治疗HUA小鼠能通过抑制XOD、GLUT9表达,继而发挥减少尿酸生成、促进尿酸排泄作用,同时有利于缓解疾病的炎症程度.
Objective:To analyze the possible mechanism of Pueraria isoflavones inhibiting XOD and GLUT9 to reduce uric acid production and promote uric acid excretion.Methods:August 2021-April 2022,a total of forty SPF male Kunming mice were divided into the healthy group(carboxymethylcellulose sodium at a dose of 250 mg/kg),the model group(HUA mice were given carboxymethylcellulose sodium at a dose of 250 mg/kg),the low group(HUA mice were given pueraria isoflavone at a dose of 125 mg/kg),HUA mice were given pueraria isoflavones at a dose of 250 mg/kg once d frequency)and the high group(HUA mice were given pueraria isoflavones at a dose of 500 mg/kg once d frequency)dosage groups,with 8 mice in each group.The contents of uric acid(SUA),urea nitrogen(BUN)and creatinine(SCr)in serum and urine of each group were compared before and after intervention(30 d).Statistical differences of xanthine oxidase(XOD)and human glucose transporter 9(GLUT9),cy-clooxygenase-2(COX-2),tumor necrosis factor(TNF-α)and interleukin-1(IL-1β)contents in renal tissues of each group after intervention(30 d)were compared.Results:After intervention,kidney inflammatory factors(COX-2,TNF-α and IL-1β)in the model group were compared.Blood and urine indexes(SUA,BUN,SCr);The contents of XOD and GLUT9 were higher than those of healthy group(P<0.05).Renal inflammatory cytokines(COX-2,TNF-α and IL-1β)in low,medium and high dose groups;Blood and urine indexes(SUA,BUN,SCr);The contents of XOD and GLUT9 were lower than those of model group,and there were low>medium>high dose groups,the comparison between the two groups had statistical significance(P<0.05).After intervention,the contents of 3 indicators in blood or urine(COX-2,TNF-α and IL-1β)all decreased compared with before intervention,and the differences in intra-group comparison were statistically significant(P<0.05).Conclusion:Pueraria isoflavones can treat HUA mice by inhibiting the expression of XOD and GLUT9,and then play a role in reducing uric acid pro-duction and promoting uric acid excretion,as well as alleviating the degree of disease inflammation.
李桐;黄春霞;梁宝月;刘晗;方少婷;张茗杰;罗婧;陈品诺;宋纪池;初晓
长沙医学院,湖南 长沙 410219
中医学
葛根异黄酮高尿酸血症黄嘌呤氧化酶人葡萄糖转运蛋白 9炎症减少生成促进排泄
Kudzu rootIsoflavoneHyperuricemiaXanthine oxidaseHuman glucose transporter 9InflammationRe-duce generationPromote excretion
《海南医学院学报》 2024 (002)
94-99 / 6
This study was supported by National Innovation and Entrepreneurship Training Program for College Students(S202010823014);Hunan Provincial College Student Innovation Training Project,No.(2021)199(S202110823045) 国家级大学生创新创业训练计划项目(S202010823014);湖南省级大学生创新训练课题,湘教通(2021)199号(S202110823045)
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