海南医学院学报2024，Vol.30Issue(2)：94-99,6.DOI:10.13210/j.cnki.jhmu.20231103.001
葛根异黄酮抑制XOD和GLUT9分别发挥减少尿酸生成、促进尿酸排泄双重途径的机制
Pueraria isoflavones inhibit XOD and GLUT9 to decrease uric acid production and promote uric acid excretion,respectively
摘要
Abstract
Objective:To analyze the possible mechanism of Pueraria isoflavones inhibiting XOD and GLUT9 to reduce uric acid production and promote uric acid excretion.Methods:August 2021-April 2022,a total of forty SPF male Kunming mice were divided into the healthy group(carboxymethylcellulose sodium at a dose of 250 mg/kg),the model group(HUA mice were given carboxymethylcellulose sodium at a dose of 250 mg/kg),the low group(HUA mice were given pueraria isoflavone at a dose of 125 mg/kg),HUA mice were given pueraria isoflavones at a dose of 250 mg/kg once d frequency)and the high group(HUA mice were given pueraria isoflavones at a dose of 500 mg/kg once d frequency)dosage groups,with 8 mice in each group.The contents of uric acid(SUA),urea nitrogen(BUN)and creatinine(SCr)in serum and urine of each group were compared before and after intervention(30 d).Statistical differences of xanthine oxidase(XOD)and human glucose transporter 9(GLUT9),cy-clooxygenase-2(COX-2),tumor necrosis factor(TNF-α)and interleukin-1(IL-1β)contents in renal tissues of each group after intervention(30 d)were compared.Results:After intervention,kidney inflammatory factors(COX-2,TNF-α and IL-1β)in the model group were compared.Blood and urine indexes(SUA,BUN,SCr);The contents of XOD and GLUT9 were higher than those of healthy group(P<0.05).Renal inflammatory cytokines(COX-2,TNF-α and IL-1β)in low,medium and high dose groups;Blood and urine indexes(SUA,BUN,SCr);The contents of XOD and GLUT9 were lower than those of model group,and there were low>medium>high dose groups,the comparison between the two groups had statistical significance(P<0.05).After intervention,the contents of 3 indicators in blood or urine(COX-2,TNF-α and IL-1β)all decreased compared with before intervention,and the differences in intra-group comparison were statistically significant(P<0.05).Conclusion:Pueraria isoflavones can treat HUA mice by inhibiting the expression of XOD and GLUT9,and then play a role in reducing uric acid pro-duction and promoting uric acid excretion,as well as alleviating the degree of disease inflammation.关键词
葛根/异黄酮/高尿酸血症/黄嘌呤氧化酶/人葡萄糖转运蛋白 9/炎症/减少生成/促进排泄Key words
Kudzu root/Isoflavone/Hyperuricemia/Xanthine oxidase/Human glucose transporter 9/Inflammation/Re-duce generation/Promote excretion分类
医药卫生引用本文复制引用
李桐,黄春霞,梁宝月,刘晗,方少婷,张茗杰,罗婧,陈品诺,宋纪池,初晓..葛根异黄酮抑制XOD和GLUT9分别发挥减少尿酸生成、促进尿酸排泄双重途径的机制[J].海南医学院学报,2024,30(2):94-99,6.基金项目
This study was supported by National Innovation and Entrepreneurship Training Program for College Students(S202010823014) （S202010823014）
Hunan Provincial College Student Innovation Training Project,No.(2021)199(S202110823045) 国家级大学生创新创业训练计划项目(S202010823014) （2021）
湖南省级大学生创新训练课题,湘教通(2021)199号(S202110823045) （2021）
