Si-SETDB1通过SPG20甲基化对肺腺癌细胞迁移侵袭的影响OACSTPCD
The Impact of Si-SETDB1 on Migration and Invasion of Lung Adenocarcinoma Cells through SPG20 Methylation
目的:检测肺腺癌中SETDB1 和SPG20 甲基化水平,探究下调SETDB1 对SPG20 甲基化及A549 细胞迁移和侵袭的影响.方法:选取60 例肺腺癌组织和正常肺组织,qRT-PCR检测 mRNA相对表达量;免疫组织化学法和Western blot检测蛋白表达量;基因甲基化水平应用焦磷酸测序法检测;构建并合成SETDB1 的小干扰RNA,脂质体介导法转染细胞,qRT-PCR 检测 mRNA 相对表达量、Wesrern blot检测蛋白表达…查看全部>>
Objective:To investigate the methylation levels of SETDB1 and SPG20 in lung adenocarci-noma and explore the impact of downregulating SETDB1 on SPG20 methylation,A549 cell migration,and in-vasion.Methods:Sixty cases of lung adenocarcinoma tissues and normal lung tissues were selected.qRT-PCR was used to detect mRNA relative expression levels,immunohistochemistry and western blot were used to detect protein expression levels,and gene methylation levels were as…查看全部>>
赵宝山;杨阳;孙光蕊;郑竞雄;梁宗英
承德医学院附属医院胸外科,河北 承德 067000河北省胸科医院临床药理试验部,河北 石家庄 050000承德医学院附属医院胸外科,河北 承德 067000承德医学院附属医院胸外科,河北 承德 067000承德医学院附属医院胸外科,河北 承德 067000
肺腺癌甲基转移酶SETDB1SPG20甲基化修饰
Lung adenocarcinomaMethyltransferaseSETDB1SPG20Methylation modifi-cation
《河北医学》 2024 (1)
8-15,8
河北省自然科学基金资助项目,(编号:H2021406045)承德市科学技术研究与发展计划项目,(编号:201904A029)
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