河北医学2024，Vol.30Issue(1)：23-28,6.DOI:10.3969/j.issn.1006-6233.2024.01.04
金银花多酚粗提物调控miR-100-5p参与非小细胞肺癌细胞NCI-H1299侵袭迁移
Regulatory Effect of Honeysuckle Polyphenol Extracts on miR-100-5p in Non-Small Cell Lung Cancer NCI-H1299 Cell Invasion and Migration
摘要
Abstract
Objective:To investigate the impact of honeysuckle polyphenol crude extract on the malig-nant biological behavior of non-small cell lung cancer(NSCLC)NCI-H1299 cells and the molecular mecha-nisms involved.Methods:NCI-H1299 cells were divided into NC group,paclitaxel group,and low,medi-um,and high-dose groups of honeysuckle polyphenol crude extract.Additionally,miR-100-5p group,miR-NC group,anti-miR-100-5p + high-dose group,and anti-miR-NC + high-dose group were established.MTT assay was used to detect cell viability,Transwell assay for cell migration and invasion,Western blot for E-Cadherin,N-Cadherin,Vimentin,and Wnt/β-catenin pathway-related protein expression,and real-time fluorescence quantitative PCR(RT-qPCR)for miR-100-5p expression.Results:After treatment with differ-ent concentrations of honeysuckle polyphenol crude extract,NCI-H1299 cell viability decreased,and the number of cell migration and invasion decreased.E-Cadherin and miR-100-5p expression levels increased,while N-Cadherin and Vimentin expression levels decreased(P<0.05).Overexpression of miR-100-5p in-creased NCI-H1299 cell viability,cell migration[(226±20.03)cells],and invasion[(171±15.03)cells],while E-Cadherin expression level decreased(0.34±0.03),and N-Cadherin(0.88±0.08)and Vi-mentin expression levels increased(P<0.05).Downregulating miR-100-5p could reverse the effects of hon-eysuckle polyphenol crude extract on NCI-H1299 cell proliferation,migration,invasion,and the Wnt/β-catenin pathway.Conclusion:Honeysuckle polyphenol crude extract may inhibit the proliferation,migration,and invasion of NSCLC NCI-H1299 cells by upregulating miR-100-5p expression,thereby suppressing the activation of the Wnt/β-catenin signaling pathway.关键词
金银花多酚粗提物/非小细胞肺癌/miR-100-5p/Wnt/β-catenin信号通路/增殖/迁移/侵袭Key words
Honeysuckle polyphenol extracts/Non-small cell lung cancer/miR-100-5p/Wnt/β-catenin signaling pathway/Proliferation/Migration/Invasion引用本文复制引用
申兴勇,白爽,李新涛,李亚红,王孝彬..金银花多酚粗提物调控miR-100-5p参与非小细胞肺癌细胞NCI-H1299侵袭迁移[J].河北医学,2024,30(1):23-28,6.基金项目
陕西省重点研发计划项目,(编号:2022SF-230) （编号:2022SF-230）
