Abstract
In order to study the in vitro bacteriostatic activity of 26 single herbs such as chebula,Lonicera japonica,Coptis chinensis against Cyprinus carpio source Aeromonas hydrophila,the assay was conducted on chebula,Lonicera japonica,Coptis chinensis and 26 other single herbs,using the boiling method to extract the active ingredients,ad-justing the herbal medicinal liquid concentrated to 1g/mL(each 1mL medicinal liquid contains 1g of raw herbs),the clinical isolation of carpio source pathogenic Aeromonas hydrophila(HT333 strain)as the test strains,the use of plate perforation method and micro two-fold dilution method to determine respectively.The diameter of the circle of inhibition and the minimum bactericidal concentration(MBC)of 26 single herbs,including chebula,Lonicera japonica,and Coptis chinensis,against the fish-derived pathogenic Aeromonas hydrophila(strain HT333)were de-termined using the plate punch method and the microdouble dilution method,respectively.The results showed ex-treme sensitivity to three species of chebula,Schisandra chinensis and Lonicera japonica,and extreme sensitivity to four species of Coptis chinensis,Sophora flavescens,Isatis tinctoria and Scutellaria baicalensis Georgi and 4 other drugs are highly sensitive,moderately sensitive to 9 drugs such as Caesalpinia sappan Linn.,Rhus chinensis Mill.and Citrus reticulata Blanco,and Corydalis bungeana,Pulsatilla chinensis,Andrographis paniculata and 3 other drugs with low sensitivity.To seven drugs such as chebula,Schisandra chinensis,Lonicera japonica,Coptis chinen-sis,Sophora flavescens,Isatis tinctoria,Scutellaria baicalensis Georgi and others the MBC ranged from 7.825mg/mL to 15.65mg/mL,to Hippophae rhamnoides Linn.,Rhus chinensis,and Cortex fraxini 3 drugs ranged from 31.25mg/mL to 62.5mg/mL,and to Citrus reticulata Blanco,Portulaca oleracea,Phellodendron chinense Schneid and 8 other drugs with MBC ranging from 125mg/mL to 250mg/mL,while it was ineffective against other drugs.This study screened chebula,Schisandra chinensis,Lonicera japonica,Coptis chinensis,Sophora flavescens,Isatis tinctoria,Scutellaria baicalensis Georgi Seven Chinese herbal medicines sensitive to Aeromonas hydrophila,the source pathogen of common carp,were used to provide a research basis for further formulas and herbal remedies for the prevention and control of Aeromonas hydrophila disease in common Cyprinus carpio.关键词
中草药/鲤(Cyprinus carpio)/嗜水气单胞菌(Aeromonas hydrophila)/体外抑菌试验Key words
Chinese herbal medicine/Cyprinus carpio/Aeromonas hydrophila/antibacterial experiment in vitro分类
水产学
