湖南中医药大学学报2024，Vol.44Issue(1)：47-53,7.DOI:10.3969/j.issn.1674-070X.2024.01.007
天名精内生真菌的分离鉴定及抗肝癌活性研究
Isolation,identification,and anti-hepatoma activity of endophytic fungi from Carpesium abrotanoides
摘要
Abstract
Objective To isolate and identify endophytic fungi from Carpesium abrotanoides and screen active strains with anti-hepatoma effects,so as to provide strain resources for the development of new anti-hepatoma lead compounds.Methods The endophytic fungi were isolated and purified from Carpesium abrotanoides by plant tissue culture and scribing method,the active strains with inhibitory effects on the proliferation of HepG2 cells were screened by MTT,and the IC50 value of the notably active endophytic fungi was calculated.Morphological and molecular identification were then applied for the identification of active strains.Results Sixty strains of endophytic fungi were isolated from Carpesium abrotanoides,among which,TMJ-03,TMJ-13,TMJ-15,TMJ-23,and TMJ-54 were active.The IC50 values of their fermentation products inhibiting the proliferation of HepG2 cells were 19.49(TMJ-C-03),31.13(TMJ-C-13),88.65(TMJ-C-15),37.42(TMJ-D-23),17.22(TMJ-D-54),and 32.72(TMJ-C-54)μg·mL-1,respectively.These five active strains have been identified to species level,namely Aspergillus terreus,Chaetomium globosum,Alternaria tillandsiae,Fusarium proliferatum,and Alternaria arborescens.Conclusion The active endophytic fungi with inhibitory effects on the HepG2 cell proliferation were screened from Carpesium abrotanoides for the first time,which can provide strain resources for the development of new anti-hepatoma drugs.关键词
天名精/内生真菌/分离鉴定/抗肝癌/HepG2细胞/活性菌株Key words
Carpesium abrotanoides/endophytic fungi/isolation and identification/anti-hepatoma/HepG2 cells/active strains分类
医药卫生引用本文复制引用
张艺,姚思凡,孙伍慧,欧阳琳,赵碧清,周小江..天名精内生真菌的分离鉴定及抗肝癌活性研究[J].湖南中医药大学学报,2024,44(1):47-53,7.基金项目
湖南省中医药科研计划项目(C2022043,A2022005-4) （C2022043,A2022005-4）
湖南省教育厅科学研究项目(19A368) （19A368）
湖南中医药大学中药学一流学科项目(2018ZYX01). （2018ZYX01）
