天名精内生真菌的分离鉴定及抗肝癌活性研究OACSTPCD

Objective To isolate and identify endophytic fungi from Carpesium abrotanoides and screen active strains with anti-hepatoma effects,so as to provide strain resources for the development of new anti-hepatoma lead compounds.Methods The endophytic fungi were isolated and purified from Carpesium abrotanoides by plant tissue culture and scribing method,the active strains with inhibitory effects on the proliferation of HepG2 cells were screened by MTT,and the IC50 value of the notably active endophytic fungi was calculated.Morphological and molecular identification were then applied for the identification of active strains.Results Sixty strains of endophytic fungi were isolated from Carpesium abrotanoides,among which,TMJ-03,TMJ-13,TMJ-15,TMJ-23,and TMJ-54 were active.The IC50 values of their fermentation products inhibiting the proliferation of HepG2 cells were 19.49(TMJ-C-03),31.13(TMJ-C-13),88.65(TMJ-C-15),37.42(TMJ-D-23),17.22(TMJ-D-54),and 32.72(TMJ-C-54)μg·mL-1,respectively.These five active strains have been identified to species level,namely Aspergillus terreus,Chaetomium globosum,Alternaria tillandsiae,Fusarium proliferatum,and Alternaria arborescens.Conclusion The active endophytic fungi with inhibitory effects on the HepG2 cell proliferation were screened from Carpesium abrotanoides for the first time,which can provide strain resources for the development of new anti-hepatoma drugs.