基于UPLC-Q-TOF-MS/MS和网络药理学探讨尖尾芋抗乳腺癌药效物质及作用机制OACSTPCD

Objective To explore the material basis and mechanism of action of Alocasia cucullata against breast cancer by UPLC-Q-TOF-MS/MS technology and network pharmacology strategies.Methods Combined with MassBank and other databases as well as existing literature research,the chemical constituents of the ethanol extract of Alocasia cucullata were identified.The anti-breast cancer targets of Alocasia cucullata were screened by TCMSP,GeneCards,and other databases.And the protein-protein interaction(PPI)network of key targets was constructed using STRING database and Cytoscape 3.9.0.Then,GO and KEGG enrichment analyses of key targets were performed by DAVID database.Finally,the interaction network of"component-gene-pathway"was constructed by Cytoscape 3.9.0.Results A total of 18 components were identified from the ethanol extract of Alocasia cucullata,including alkaloids(1,3,10,12),phenylpropanoids(2,8,18),and flavonoids(6,7,9,11,15),etc.Based on the identified compounds,429 potential targets were obtained by network pharmacology.In addition,ten core targets such as PIK3CA,PIK3R1,and MAPK1 were found by PPI analysis.Enrichment analysis revealed that these core targets may exert anti-breast cancer effects by regulating cancer pathways.Moreover,the interaction network of"component-gene-pathway"showed that the alkaloid component berberine and the flavonoid components kaempferol and luteolin may be the main active components of the ethanol extract of Alocasia cucullata responsible for pharmacological effects,and their mechanism was related to apoptosis.Conclusion This study preliminarily explored that the anti-breast cancer active components of the ethanol extract of Alocasia cucullata were alkaloids and flavonoids,and their mechanism of action was related to apoptosis,which provides new ideas and clues for further research on the material basis and mechanism of action of the ethanol extract of Alocasia cucullata against breast cancer.