湖南中医药大学学报2024，Vol.44Issue(1)：77-85,9.DOI:10.3969/j.issn.1674-070X.2024.01.012
基于UPLC-Q-TOF-MS/MS和网络药理学探讨尖尾芋抗乳腺癌药效物质及作用机制
Anti-breast cancer pharmacodynamic substances and mechanism of action of Alocasia cucullata based on UPLC-Q-TOF-MS/MS and network pharmacology
摘要
Abstract
Objective To explore the material basis and mechanism of action of Alocasia cucullata against breast cancer by UPLC-Q-TOF-MS/MS technology and network pharmacology strategies.Methods Combined with MassBank and other databases as well as existing literature research,the chemical constituents of the ethanol extract of Alocasia cucullata were identified.The anti-breast cancer targets of Alocasia cucullata were screened by TCMSP,GeneCards,and other databases.And the protein-protein interaction(PPI)network of key targets was constructed using STRING database and Cytoscape 3.9.0.Then,GO and KEGG enrichment analyses of key targets were performed by DAVID database.Finally,the interaction network of"component-gene-pathway"was constructed by Cytoscape 3.9.0.Results A total of 18 components were identified from the ethanol extract of Alocasia cucullata,including alkaloids(1,3,10,12),phenylpropanoids(2,8,18),and flavonoids(6,7,9,11,15),etc.Based on the identified compounds,429 potential targets were obtained by network pharmacology.In addition,ten core targets such as PIK3CA,PIK3R1,and MAPK1 were found by PPI analysis.Enrichment analysis revealed that these core targets may exert anti-breast cancer effects by regulating cancer pathways.Moreover,the interaction network of"component-gene-pathway"showed that the alkaloid component berberine and the flavonoid components kaempferol and luteolin may be the main active components of the ethanol extract of Alocasia cucullata responsible for pharmacological effects,and their mechanism was related to apoptosis.Conclusion This study preliminarily explored that the anti-breast cancer active components of the ethanol extract of Alocasia cucullata were alkaloids and flavonoids,and their mechanism of action was related to apoptosis,which provides new ideas and clues for further research on the material basis and mechanism of action of the ethanol extract of Alocasia cucullata against breast cancer.关键词
尖尾芋醇提物/抗乳腺癌/超高效液相色谱-四级杆飞行时间串联质谱/网络药理学/相互作用/富集分析Key words
ethanol extract of Alocasia cucullata/anti-breast cancer/UPLC-Q-TOF-MS/MS/network pharmacology/in-teraction/enrichment analysis分类
医药卫生引用本文复制引用
王鹏,陈娅,彭江丽,彭求贤..基于UPLC-Q-TOF-MS/MS和网络药理学探讨尖尾芋抗乳腺癌药效物质及作用机制[J].湖南中医药大学学报,2024,44(1):77-85,9.基金项目
国家自然科学基金项目(81973593) （81973593）
湖南省自然科学基金-科药联合项目(2022JJ80087) （2022JJ80087）
湖南中医药大学研究生广东一方制药有限公司科研基金项目(2021YF01) （2021YF01）
湖南中医药大学中药学一流学科基金资助项目(校行科字[2018]3号)资助. （校行科字[2018]3号）
