中医药导报2023，Vol.29Issue(12)：7-11,5.DOI:10.13862/j.cn43-1446/r.2023.12.002
益气、活血、化痰法不同组合对COPD大鼠炎症反应及气道黏液高分泌的干预作用
The Intervention of Different Combinations of Yiqi(益气),Huoxue(活血)and Huatan(化痰)Treatment on the Inflammatory Reaction and Mucosal Hypersecretion of Airway Mucus in COPD Rats
摘要
Abstract
Objective:To observe the effects of different treatment regimens of Yiqi,Huoxue and Huatan on the levels of inflammatory cytokines IL-8 and TNF-α,as well as the moderation effect on aquaporin5(AQP5)and mucin5AC(MUC5AC)of alveolar lavage fluid(BALF)in rats with chronic obstructive pulmonary disease(COPD).Methods:Totally 54 SD rats were randomly divided into blank group,model group,Yiqi Huatan group,Yiqi Huoxue group,Huoxue Huatan group and Yiqi Huoxue Huatan group,9 rats in each group.Except for the blank group,COPD rats were established by tracheal instillation of cigarette smoke and lipopolysaccharide in the other groups.The rats were killed after 28 days on the day of modeling,and the serum,BALF and left lung tissue were collected.Lung tissue pathology was observed according to histopathology(hematoxylin-eosin)staining.And enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of AQP5 and MUC5AC in BALF and serum IL-8 and TNF-α.Results:Compared with the model group,the lung tissue and airway structure of rats were relatively intact,and the degree of inflammation infiltration was milder in Yiqi Huoxue group,Yiqi Huatan group,Huoxue Huatan group and Yiqi Huoxue Huatan group.After Chinese medicine intervention,the lung tissue pathological damage in COPD model rats was improved to a certain extent.Compared with the blank group,the levels of serum IL-8 and TNF-α were significantly increased in model group(P＜0.05).Compared with the model group,the levels of serum IL-8 and TNF-αwere significantly decreased in Yiqi Huoxue group,Yiqi Huatan group,Huoxue Huatan group and Yiqi Huoxue Huatan group(P＜0.05).There was no significant difference in TNF-α levels among the Chinese medicine intervention groups(P＞0.05).Compared with the blank group,the AQP5 level in BALF was significantly decreased in model group(P＜0.05),while the MUC5AC level was significantly increased(P＜0.05).Compared with the model group,the levels of AQP5 in BALF were signifi-cantly increased in Yiqi Huoxue group,Yiqi Huatan group,Huoxue Huatan group and Yiqi Huoxue Huatan group(P＜0.05),and the levels of MUC5AC were significantly decreased(P＜0.05).Conclusion:The curative effect of different combination therapy of Yiqi,Huoxue and Huatan on COPD may be closely related to inhibiting inflammation reaction and relieving airway mucus hypersecretion.关键词
慢性阻塞性肺疾病/气道黏液高分泌/炎症反应/益气活血化痰/大鼠Key words
chronic obstructive pulmonary disease/mucus hypersecretion/inflammatory reaction/Yiqi Huoxue Huatan/rat分类
医药卫生引用本文复制引用
董洪珍,黄慧婷,周游,张伟..益气、活血、化痰法不同组合对COPD大鼠炎症反应及气道黏液高分泌的干预作用[J].中医药导报,2023,29(12):7-11,5.基金项目
国家中医药管理局基金项目(JDZX2012167) （JDZX2012167）
广东省中医药局基金项目(20212058) （20212058）
