花生学报2023，Vol.52Issue(4)：7-18,12.DOI:10.14001/j.issn.1002-4093.2023.04.002
钙肥、铁肥对长期连作花生土壤真菌群落结构的影响
Effect of Calcium and Iron Fertilizer on Fungal Community Structure in Long-Term Continuous Cropped Peanut Soil
摘要
Abstract
The continuous cropping obstacle is a prominent problem that restricts the sustainable development of the peanut industry in China.The analysis of the mechanism of peanut continuous cropping obstacles can provide theoretical basis and technical support for overcoming and alleviating the obstacles of peanut continuous cropping.To explore the effects of calcium and iron fertilizer on the distribution of fungal community in continuous cropped peanut soil,the calcium and iron fertilizer treated soil was analyzed using high-throughput sequencing technology,UPGMA methods,and heat-map analysis.The UPGMA analysis showed that applying calcium fertilizer or iron fertilizer can affect the fungal community structure of peanut soil,and that the influence of the fungal community struc-ture of long-term continuous cropped peanut soil was greater than that of peanut varieties.Heatmap a-nalysis showed that the fungal community structure varied significantly across taxonomic levels in the calcium and iron fertilizer treated soils compared with the control soil.At the phylum level,the abun-dance of Ascomycota and Mucoromycota significantly increased in the soil of both peanut varieties,while Basidiomycota decreased.The abundance of Chytridomycota decreased in Huayu20-cropped soil,but increased in Huayu26-cropped soil.The influence of calcium fertilizer was significantly stronger than that of iron fertilizer.At the genus level,the analysis further showed that calcium and iron fertil-izer can significantly change the fungal community structure in peanut soil.Calcium fertilizer can sig-nificantly increase the overall abundance of dominant fungi in peanut soil,and the increase was signifi-cantly higher in Huayu26-cropped soil than in Huayu20-cropped soil.The relative abundance of domi-nant fungal was also increased in Huayu26-cropped soil,while slightly reduced in Huayu20-cropped soil under iron fertilizer treatment.The results indicated that the content of calcium and iron in contin-uous cropped peanut soil can affect the fungal community structure,and the application of calcium and iron fertilizer can significantly increase the overall abundance of dominant fungi in peanut cropped soil.This study opened a new perspective for further research on the difference mechanism of different de-grees of continuous cropping tolerance in different peanut varieties.关键词
花生/连作/钙肥/铁肥/真菌群落结构Key words
peanut/continuous cropping/calcium fertilizer/iron fertilizer/fungal community structure分类
农业科技引用本文复制引用
迟晓元,陈娜,陈明娜,许静,潘丽娟,姜骁,殷祥贞,赵旭红,王欢,史晓梅..钙肥、铁肥对长期连作花生土壤真菌群落结构的影响[J].花生学报,2023,52(4):7-18,12.基金项目
新疆维吾尔自治区重大科技专项(2022A02008-3) （2022A02008-3）
泰山学者工程专项(tsqn201812121) （tsqn201812121）
财政部和农业农村部:国家现代农业产业技术体系(CARS-13) （CARS-13）
山东省自然科学基金项目(ZR2021QC172) （ZR2021QC172）
广东省重点领域研发计划项目(2020B020219003) （2020B020219003）
山东省重点研发计划(农业良种工程)(2020LZGC001) （农业良种工程）
山东省重点研发计划项目(乡村振兴科技创新提振行动计划)(2022TZXD0031) （乡村振兴科技创新提振行动计划）
