家畜生态学报2024，Vol.45Issue(1)：22-28,7.DOI:10.3969/j.issn.1673-1182.2024.01.004
不同青贮饲料对奶牛瘤胃液微生物多样性的影响
Effects of Different Silage on Microbial Diversity in Rumen Fluid of Dairy Cows
摘要
Abstract
In order to better exploit and utilize sweet sorghum silage,Illumina MiSeq 2 × 250 system was used to perform high-throughput sequencing of the V3-V4 region of bacterial 16SrDNA.The bacterial diversity and community structure of rumen fluid samples from high sugar sweet sorghum silage(HSS),low sugar sweet sorghum silage(LSS)and maize silage(MZS)were analyzed.The results showed that the microbial richness of rumen fluid extracted from HSS and MZS was similar and higher than in LSS.The proportion of Bacteroidetes in HSS and MZS was the largest,which was significantly higher than that in LSS(P＜0.05),accounting for 39.65％and 40.51％,respectively.Firmicutes accounted for 29.67％and 32.87％,which were lower than in LSS.According to the analysis of bacterial genera,Prevotella and Ru-minococcus were dominant bacteria in three rumen fluids.There are many commonalities between HSS and MZS rumen fluids,and the richness of bacterial genera is higher than that of LSS.The results showed that HSS can be used to replace MZS to feed cows.关键词
高通量测序/瘤胃微生物/甜高粱/多样性/奶牛Key words
high-throughput sequencing/rumen microbes/sweet sorghum/diversity/cows分类
农业科技引用本文复制引用
周斐然,赵龙飞,张梦圆,张苏江..不同青贮饲料对奶牛瘤胃液微生物多样性的影响[J].家畜生态学报,2024,45(1):22-28,7.基金项目
国家自然科学基金项目(31160472,31260565) （31160472,31260565）
哈密市科学技术项目(hm2021kj10) （hm2021kj10）
