激光生物学报2023，Vol.32Issue(6)：502-516,15.DOI:10.3969/j.issn.1007-7146.2023.06.003
CRISPR/Cas:可应用于病毒检测和抗病毒治疗的前沿技术
CRISPR/Cas:Promising Technology for Virus Detection and Antiviral Therapy
摘要
Abstract
Frequent viral epidemics in recent years urge the development of efficient and convenient techniques for virus de-tection and antiviral drugs development.As the most popular gene-editing tools,engineered systems based on clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)possess high specificity and efficiency in nucleic acid targeting and cleavage,which have also been widely used in virological research and medical practice.This re-view briefly introduces the features of three most commonly used CRISPR/Cas systems,including Cas9,Cas12 and Cas13,and comprehensively concludes their application in virus detection and antiviral therapeutics.For virus detection,Cas9 enhances the sensitivity and accuracy of biosensor detection by combining it with biosensors such as fluorescent sensors,electrochemical sen-sors,and lateral flow chromatography.As for Cas12 and Cas13,multiple technologies have been developed to detect DNA and RNA viruses based on their trans-cleavage activity,such as SHERLOCK and DETECTR.As CRISPR/Cas-based antiviral tech-niques,Cas9 has been employed to cleave viral DNA,including genomes of DNA viruses and intermediate DNA of retroviruses,while Cas13 has been used to target viral RNA,including genomes of RNA viruses and viral mRNA.Although CRISPR/Cas systems have shown multiple advantages in sensitivity,efficiency and convenience,they still bear some limitations,such as off-target effect,immunogenicity and carcinogenicity.In summary,this manuscript provides an overview of current progress in the application of different CRISPR/Cas systems as promising tools in virus detection and antiviral therapy.关键词
CRISPR/Cas系统/病毒检测/抗病毒治疗/生物传感器Key words
CRISPR/Cas system/virus detection/antiviral therapy/biosensor分类
生物科学引用本文复制引用
梁锐敏,邹盈盈,邱烨..CRISPR/Cas:可应用于病毒检测和抗病毒治疗的前沿技术[J].激光生物学报,2023,32(6):502-516,15.基金项目
国家自然科学基金面上项目(32270170) （32270170）
国家自然科学基金青年项目(81902070). National Natural Science Foundation of China(32270170),Young Scientists Fund of the National Natural Science Foundation of China(81902070). （81902070）
