激光生物学报2023，Vol.32Issue(6)：525-536,12.DOI:10.3969/j.issn.1007-7146.2023.06.005
烟草脉斑驳病毒侵染本氏烟的转录组研究
Transcriptom Analysis of Nicotiana benthamiana Infeced by Tobacco Vein Mottling Virus
摘要
Abstract
In order to study the molecular mechanism of tobacco vein mottling virus(TVMV)infection of Nicotiana ben-thamiana,this study established a TVMV transcriptome database,excavated disease-resistant genes,metabolic and signaling pathways,and used the Illumina Novaseq 6000 high-throughput sequencing platform to perform transcriptome sequencing and bioinformatics analysis Nicotiana benthamiana infected with TVMV.We used software such as R package:edge R to analyze differentially expressed genes related to disease resistance,and conducted gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis on differentially expressed genes in the KEGG.A total of 4 593 differen-tially expressed genes were obtained based on the transcriptome sequencing results of TVMV infection,among which the 3 564 genes were up-regulated and 1 029 genes were down-regulated.A total of 10 differentially expressed genes related to disease resistance were screened,6 were up-regulated and 4 were down-regulated.A total of 50 functional groups have been annotated in the GO database in three categories:biological processes,cell components and molecular functions.Among them,in the third class of molecular functions,the differentially expressed genes involved in protein binding function and ATP binding function were most significant,there were 501 and 453 differential genes,respectively.KEGG was enriched in 20 pathways,annotation to ribosome pathway has the highest degree of differential gene enrichment.307 differentially expressed genes were more sig-nificant.Protein binding,ATP binding,ribosome,eukaryotic ribosome biological reaction,DNA replication and other pathways may play an important role in the regulation of TVMV.The analysis of differentially expressed genes screened in transcriptome has laid an important foundation for further research on the resistance of Nicotiana benthamiana to TVMV virus.关键词
烟草脉斑驳病毒/转录组/KEGG通路/本氏烟/生物信息学分析Key words
tobacco vein mottle virus/transcriptome/KEGG pathway/Nicotiana benthamiana/bioinformatics analysis分类
生物科学引用本文复制引用
张荠丹,孙振琪,岳建英,王海娟,赵倩倩,杨向东,赵明敏..烟草脉斑驳病毒侵染本氏烟的转录组研究[J].激光生物学报,2023,32(6):525-536,12.基金项目
国家自然科学基金项目(31860489). （31860489）
