激光生物学报2023，Vol.32Issue(6)：548-553,560,7.DOI:10.3969/j.issn.1007-7146.2023.06.007
一株褐家鼠戊型肝炎病毒的发现鉴定及系统发生分析
Identification and Phylogenetic Analysis of a Strain of Hepatitis E Virus from Rattus norvegicus
摘要
Abstract
Hepatitis E virus(HEV)harbored by small mammals in Dali City,Yunnan Province was investigated.The samples were detected by nested RT-PCR and whole genomes were sequenced by high-throughput sequencing in the positive HEV sam-ples.Bioinformatics was analyzed by the soft-wares of MEGA-X,BESTv1.10.4 and Geneious.A total of 114 small mammals from 6 genera and 7 species were screened in this study.The positivity rate of HEV in Norway rat(Rattus norvegicus)was 8.00%(2/25),and the whole genome of one HEV strain of No.DL147 was obtained,which was the HEV-C1 genotype,and had the highest homology to HEV in the serum of a patient with hepatitis E in Hong Kong,with a nucleotide level of 95.21%.The earliest divergence between the two strains was about 2003.The study confirmed the presence of HEV-C1 infection in Norway rat in Dali City,Yunnan Province,which is highly homologous to the recently reported human emerging HEV and has a relative-ly recent phylogenetic relationship,and it is instructive for prevention and control of hepatitis E.关键词
戊型肝炎病毒/人兽共患病/啮齿动物/遗传分析/高通量测序Key words
hepatitis E virus/zoonosis/rodents/genetic analysis/high throughput sequencing分类
医药卫生引用本文复制引用
宗利东,殷红敏,杨银,赵维玉,徐粉慧,韩培钰,崔凤灵,何明仙,张云智..一株褐家鼠戊型肝炎病毒的发现鉴定及系统发生分析[J].激光生物学报,2023,32(6):548-553,560,7.基金项目
国家自然科学基金项目(U2002218,81874274) （U2002218,81874274）
云南省高层次卫生计生技术人才培养项目(L-2017027) （L-2017027）
大理大学博士科研启动费项目(KYBS2018004) （KYBS2018004）
大理大学人兽共患病跨境防控与检疫创新团体项目(ZKPY2019302). （ZKPY2019302）
