中国当代医药2024，Vol.31Issue(1)：10-14,5.
LncRNA-CASC2竞争性结合miR-193a-5p调控GSDMD焦亡通路逆转非小细胞肺癌顺铂耐药的分子机制
Molecular mechanisms of LncRNA-CASC2 competitively binding miR-193a-5p regulating GSDMD scorch death pathway reversing Cisplatin re-sistance in non-small cell lung cancer
摘要
Abstract
Objective To explore the molecular mechanism of the competitive binding of LncRNA-CASC2 to miR-193a-5p in regulating GSDMD focal death pathway to reverse cisplatin resistance in non-small cell lung cancer.Methods Non-small cell lung cancer(NSCLC)cell line A549 was selected to establish a drug-resistant strain A549/DDP,and the sensitivity of the cell to cisplatin was detected by CCK8 to verify whether the drug-resistant cell line was success-fully established.The expression of CASC2 in A549 and A549/DDP cells and its effect on the expression of GSDMD2 and miR-193a-5p were detected by PCR.The binding sites of LncRNA-CASC2 and miR-193a-5p,miR-193a-5p and GSD-MD were predicted by bioinformatics software.The target relationship between CASC2 and miR-193a-5p and miR-193a-5p and GSDMD was analyzed by luciferase reporter gene,and the competitive binding of LncRNA-CASC2 to miR-193a-5p was detected by Western blot.Results The semi-inhibitory concentration of A549/DDP cells was more than 10 times that of A549 cells,indicating that the drug-resistant cell line A549/DDP had been successfully constructed.The expression level of CASC2 in A549/DDP was lower than that in A549(P＜0.05).CASC2 mimics inhibited the expression of miR-193a-5p in cells,while GSDMD expression was up-regulated(P＜0.05).There was a significant targeting relationship between CASC2 and miR-193a-5p,and between miR-193a-5p and GSDMD(P＜0.05).Conclusion The low expression of LncRNA-CASC2 and miR-193a-5p in NSCLC cells and the high expression of GSDMD are closely related to the chemotherapy resistance of NSCLC.The interaction of LncRNA-CASC2 and miR-193a-5p regulates GSDMD and promotes the drug resistance of tumor cells.关键词
LncRNA-CASC2/miR-193a-5p/GSDMD/耐药Key words
LncRNA-CASC2/miR-193a-5p/GSDMD/Drug resistance分类
医药卫生引用本文复制引用
罗世程,肖冬平,黄秀玲,左炜,胡向东,邓志辉..LncRNA-CASC2竞争性结合miR-193a-5p调控GSDMD焦亡通路逆转非小细胞肺癌顺铂耐药的分子机制[J].中国当代医药,2024,31(1):10-14,5.基金项目
江西省卫生健康委科技计划项目(202211574). （202211574）
