兰州大学学报（医学版）2023，Vol.49Issue(11)：26-32,7.DOI:10.13885/j.issn.1000-2812.2023.11.004
白藜芦醇调控TGF-β1/Smad信号通路对脑胶质瘤细胞增殖、凋亡的影响
Effects of resveratrol on proliferation and apoptosis of glioma cells by regulating TGF-β1/Smad signaling pathway
摘要
Abstract
Objective To investigate the effect of resveratrol on the proliferation and apoptosis of glioma cells by regulating transforming the growth factor-β1(TGF-β1)/Smad signaling pathway.Methods Human glioma T98G cells were divided into a control group,25 μ mol/L resveratrol group,50 μ mol/L resveratrol group,100 μmol/L resveratrol group,5-fluorouracil(5-FU)group,LY2109761(LY)group and SRI-011381(SRI)group for 24 hours.Cell viability,proliferation rate,apoptosis,adhesion,invasion ability,expression of Cy-clinD1,Caspase-3 and TGF-β1/Smad signaling pathway and related protein expression levels were analyzed by cell count,5-ethynyl-2'deoxyuracil,Hoechst 33258 staining,adhesion assay,Transwell chamber and West-ern blotting.Results The cell viability of 50,100 μmol/L resveratrol groups and the 5-FU group was lower than that of the control group(P<0.05).Compared with the control group,the cell proliferation rate,adhesion,invasion ability and protein levels of CyclinD1,TGF-β1 and p-Smad3 in the 50 μmol/L resveratrol group and 5-FU group were decreased,while the apoptosis rate,Caspase-3 and Smad7 protein levels increased(P<0.05).The changes in the above indexes in the LY group were further strengthened(P<0.05),while those of the SRI group had an opposite trend to the LY group(P<0.05).Conclusion Resveratrol can inhibit the proliferation,adhesion and invasion of human glioma T98G cells and induce their apoptosis,whose mecha-nism may be connected to the inhibition of the activation of the TGF-β1/Smad signaling pathway.关键词
白藜芦醇/脑胶质瘤/增殖/凋亡/黏附/侵袭/信号通路Key words
resveratrol/glioma/proliferation/apoptosis/adhesion/invasion/signaling pathway分类
医药卫生引用本文复制引用
王勇,吴运桥,邓任强,靳宜静..白藜芦醇调控TGF-β1/Smad信号通路对脑胶质瘤细胞增殖、凋亡的影响[J].兰州大学学报（医学版）,2023,49(11):26-32,7.基金项目
海南省卫生健康行业科研资助项目(21A200325) （21A200325）
