生物质化学工程2024，Vol.58Issue(1)：18-26,9.DOI:10.3969/j.issn.1673-5854.2024.01.003
靛蓝提取物中靛蓝和靛玉红的分析方法比较
Comparation of Three Methods of Analytical Methodology of Indigo and Indirubin in Different Indigo Extracts
摘要
Abstract
Using indigo paste as raw material,indigo acidification substances,glucose reducing substances,and ethyl acetate extractives were prepared using three processes of hydrochloric acid acidification,glucose reduction,and ethyl acetate extraction,respectively.High performance liquid chromatography(HPLC),ultraviolet visible spectrophotometry(UV),and dual wavelength ultraviolet spectrophotometry(dW-UV)were established to analyze the contents of indigo and indirubin in samples from three processes.The optimal analysis methods for each of three samples were determined through Kolmogorov Smirnov test,comparison of Bland Altman deviation plots,and Spearman test.The results showed that the established HPLC,UV,and dW-UV methods were simple,accurate,highly sensitive,stable,and reproducible.Three methods for detecting the content of indigo extract components all followed the normal distribution,and there were significant differences between each the two measured values.The UV method was more suitable for analyzing the content of indigo in indigo acidified substances and indigo reducing substances.HPLC method was more suitable for measuring the content of indigo and indirubin in ethyl acetate extract.关键词
靛蓝/靛玉红/统计分析/HPLC法/UV光谱法Key words
indigo/indirubin/statistic analysis/HPLC method/UV spectrometry分类
化学化工引用本文复制引用
王成章,颜洋洋,周昊,刘丹阳,商士斌,张华兴..靛蓝提取物中靛蓝和靛玉红的分析方法比较[J].生物质化学工程,2024,58(1):18-26,9.基金项目
中国林科院中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2021ZC003) （CAFYBB2021ZC003）
贵州科技支撑项目(黔科合支撑[2022]-159) （黔科合支撑[2022]-159）
