临床口腔医学杂志2024，Vol.40Issue(1)：3-7,5.DOI:10.3969/j.issn.1003-1634.2024.01.002
LncRNA DANCR通过负调控miR-216a表达参与牙周膜干细胞成骨分化和迁移
LncRNA DANCR participates in osteogenic differentiation and migration of periodontal ligament cells through negative regulation of miR-216a expression
摘要
Abstract
Objective:To explore the effect of long non-coding RNA differentiation antagonizing non-protein coding RNA(lncRNA DANCR)on the osteogenic differentiation and migration of human periodontal ligament stem cells(hPDLSCs)and its underlying mechanism.Methods:The protein expression of osteogenic markers alkaline phosphatase(ALP),osteocal-cin(OCN)and Runt-related transcription factor 2(RUNX2)was detected by Western blot.The levels of miR-216a-5p and DANCR were detected by qRT-PCR.The relationship between miR-216a-5p and DANCR was assessed by luciferase reporter gene,RNA immunoprecipitation.Transwell assay was used to detect cell migration ability.Results:The levels of DANCR ex-pression decreased and miR-216a-5p expression increased after PDLSCs were stimulated by osteogenic differentiation induc-tion medium.DANCR overexpression or miR-216a-5p knockdown both reduced the protein expression of ALP,OCN and RUNX2 in PDLSCs,and inhibited ALP activity and cell migration ability.There was a targeting relationship between DANCR and miR-216a-5p.Conclusion:DANCR may participate in the osteogenic differentiation and migration of PDLSCs by regula-ting the expression of miR-216a.关键词
牙周炎/LncRNA DANCR/miR-216a/牙周膜细胞Key words
Periodontitis/LncRNA DANCR/miR-216a/Periodontal ligament cells分类
医药卫生引用本文复制引用
任燕,张雪,白静,李月辉..LncRNA DANCR通过负调控miR-216a表达参与牙周膜干细胞成骨分化和迁移[J].临床口腔医学杂志,2024,40(1):3-7,5.基金项目
河北省自然医学科学基金项目(编号20230540) （编号20230540）
