临床口腔医学杂志2024，Vol.40Issue(1)：8-12,5.DOI:10.3969/j.issn.1003-1634.2024.01.003
miR-548d-5p通过TLR2/Myd88/NF-κB信号通路促进牙周膜干细胞细胞增殖和成骨分化的研究
miR-548d-5p contributes to cell proliferation and osteogenic differentiation via TLR2/Myd88/NF-κB axis in periodontal ligament stem cells
卢妍君 1李文霞 2于飞2
作者信息
- 1. 重庆医科大学第一临床学院 重庆 400016
- 2. 华中科技大学同济医学院附属同济医院口腔医学中心 湖北 武汉 430030
- 折叠
摘要
Abstract
Objective:To explore the effect and mechanism of TLR2 negative upstream regulatory factor miR-548d-5p on the proliferation and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs).Methods:Construct TLR2 overexpression vector TLR2 OE.TLR2 OE and miR-548d-5p mimic were transfected into hPDLSCs respectively.The transfection efficiency was evaluated by RT-qPCR.Proliferation ability of hPDLSCs was detected by CCK-8 assay and clone formation experiment.Alizarin red staining and Western blotting were used to determine the expression level of OCN protein and evaluate the osteogenic differentiation ability of hPDLSCs.TLR2/MyD88/NF-κB signaling pathway molecules Myd88 and NF-κB expression levels were determined by Western blot.Results:The survival rate,clone formation numbers and num-bers of mineralized nodules formed by hPDLSCs were significantly reduced after transfection with TLR2 OE,while the ex-pression levels of Myd88 and NF-κB molecules were significantly incresed(P＜0.05).The survival rate,clone formation num-bers and numbers of mineralized nodules formed by hPDLSCs were significantly incresed after transfection with miR-548d-5p mimic,while the expression levels of Myd88 and NF-κB molecules were significantly reduced(P＜0.05).Compared with the control group,there was no significant difference in survival rate,clone formation numbers,numbers of mineralized nodules formed by hPDLSCs and Myd88 and NF-κB expression levels in TLR2 OE and miR-548d-5p mimic co-transfection group.Conclusion:Overexpression of TLR2 inhibited the proliferation and osteogenic differentiation of hPDLSCs.miR-548d-5p en-hanced the proliferation and osteogenic differentiation ability of hPDLSCs.MiR-548d-5p could antagonize the effect of TLR2 on the proliferation and osteogenic differentiation of hPDLSCs through regulating TLR2/Myd88/NF-κB signaling pathway.关键词
Toll样受体2/牙周膜干细胞/miRNAKey words
Toll like receptor 2/Periodontal ligament stem cells/miRNA分类
生物科学引用本文复制引用
卢妍君,李文霞,于飞..miR-548d-5p通过TLR2/Myd88/NF-κB信号通路促进牙周膜干细胞细胞增殖和成骨分化的研究[J].临床口腔医学杂志,2024,40(1):8-12,5.
