临床眼科杂志2023，Vol.31Issue(6)：555-560,6.DOI:10.3969/j.issn.1006-8422.2023.06.015
S1PR1激活PI3K/Akt保护高糖环境下视网膜血管内皮细胞的生物学功能
S1PR1 protects the biological function of retinal vascular endothelial cells under high glucose condition via ac-tivating PI3K/Akt
摘要
Abstract
Objective To study the effect of sphingosine 1-phosphate receptor 1(S1PR1)on the migration and tube formation of human retinal vascular endothelial cells(HREC)under high glucose condition,and to further explore its mechanism.Methods A prospective case-control study.HREC were randomly divided into control group,S1PR1 overex-pression group,high glucose+control group and high glucose+S1PR1 overexpression group.Cell scratch assays and tran-swell were used to detect the migration of HREC,tube formation assay was used to detect the tube formation of HREC,and Western blot was used to detect the expression of PI3K/Akt pathway-related protein molecules.Results Compared with the control group,the wound healing percentage,the cell number of migration,the number of branch points and the total length of the tubules in the high glucose+control group were significantly reduced(all P＜0.01).While compared with the high glucose+control group,there were significantly increased in the high glucose+S1PR1 overexpression group(all P＜0.01).There were no significant differences in the total protein expressions of PI3K and Aktamong the groups(all P＞0.05),but there were significantly different in the expressions of p-PI3K and p-Akt(all P＜0.01).Compared with the control group,the expressions of p-PI3 K and p-Akt in the high glucose+control group were significantly decreased(all P＜0.01).While compared with the high glucose+control group,their expressions in the high glucose+S1PR1 overex-pression group were significantly increased(all P＜0.01).Conclusions S1PR1 protects the migration and tube-formation ability of HREC under high glucose condition by activating the PI3K/Akt pathway.关键词
1-磷酸鞘氨醇受体/视网膜血管内皮细胞/迁移/成管/高糖/PI3K/AktKey words
S1PR1/Retinal endothelial cell/Migration/Tube formation/High glucose/PI3K/Akt引用本文复制引用
范玲玲,刘伦..S1PR1激活PI3K/Akt保护高糖环境下视网膜血管内皮细胞的生物学功能[J].临床眼科杂志,2023,31(6):555-560,6.基金项目
安徽省自然科学基金(1908085QH381) （1908085QH381）
