生态与农村环境学报2023，Vol.39Issue(12)：1646-1656,11.DOI:10.19741/j.issn.1673-4831.2023.0132
伸展摇蚊(Chironomus tentans)转录组测序及胰岛素信号通路关键基因序列分析
Transcriptome Sequencing and Sequence Analysis of Key Genes in the Insulin Signaling Pathway of Chironomus tentans
摘要
Abstract
To explore the endocrine disrupting gene markers of invertebrates based on the insulin signal pathway,this study selected Chironomus tentans as the model organism,identified the key genes in the insulin signal pathway on the ba-sis of transcriptome sequencing,and carried out the full-length amplification of cDNA and the multi-species comparison of amino acid sequences for the upstream genes such as InR,Pdk,Akt and FoxO.Based on the cDNA full-length sequence,the InR,Pdk,Akt and FoxO genes of Chironomus tentans can encode protein sequences containing 1 436,522,520 and 470 amino acids,respectively.The sequence homology comparison and phylogenetic tree analysis results verified its evolu-tionary conservatism.The qRT-PCR results of the relative expression of genes in Ⅰ-Ⅳ instar larvae,pupae,female adults and male adults show that the expression trend of InR,Pdk,Akt and FoxO in C.tentans was similar.The peaks of gene ex-pression in Ⅰ and Ⅳ instar indicate that insulin signaling induces faster growth and development rates in Ⅰ and Ⅳ instar lar-vae.The expression of InR,Pdk,Akt,and FoxO in female adults was significantly higher than that in males,which may be related to the involvement of insulin signaling in the development of the female reproductive system.关键词
胰岛素信号通路/转录组学/序列分析/qRT-PCRKey words
insulin signaling pathway/transcriptomics/sequential analysis/qRT-PCR分类
资源环境引用本文复制引用
张成林,古文,汪贞,石利利,王蕾,杨家新..伸展摇蚊(Chironomus tentans)转录组测序及胰岛素信号通路关键基因序列分析[J].生态与农村环境学报,2023,39(12):1646-1656,11.基金项目
生态环境部南京环境科学研究所创新团队建设项目(ZX2023QT003) （ZX2023QT003）
