南京农业大学学报2024，Vol.47Issue(1)：69-77,9.DOI:10.7685/jnau.202208014
牦牛源短小芽胞杆菌TS1的分离鉴定及其生物学特性研究
Isolation,identification and biological characterization of a strain of Bacillus pumilus TS1 from yak cattle
摘要
Abstract
[Objectives]The aim of this experiment was to study the isolation and identification,resistance to common antimicrobial drugs and antibacterial activity of a strain of Bacillus subtilis of yak origin(named:TS1),and to explore the feasibility of its application as a probiotic additive in animal husbandry.[Methods]The fresh manure of healthy cattle was treated with water bath at 100℃for 10 min to screen the resistant Bacillus,and then the morphological,physiological and biochemical characteristics and 16S rRNA sequence analysis were integrated to identify them,and their biological characteristics were studied by measuring growth curves,acid and bile salt resistance tests,drug sensitivity tests and inhibition tests,and their possible production of the types of antibacterial substances they may produce.[Results]TS1 was Bacillus pumilus with acid-resistant and bile salt-resistant growth characteristics;TS1 was sensitive to 12 common antibacterial drugs and had inhibitory effects on Escherichia coli,Salmonella,Staphylococcus aureus and Streptococcus suis.Sequencing analysis showed that this strain could encode a novel antimicrobial peptide with 48.90%homology to Amylocyclicin,and thus exerted its antimicrobial activity.[Conclusions]This Bacillus pumilus TS1 has certain resistance and potential probiotic ability and broad-spectrum antibacterial effect,and has low risk of antibiotic resistance transmission in the livestock and poultry intestinal microbial system,and is suitable as a probiotic additive class antibiotic replacement product.关键词
短小芽胞杆菌/分离鉴定/抑菌/抗生素替代品:益生菌添加剂Key words
Bacillus pumilus/isolation and identification/bacterial inhibition/alternative to antibiotics/probiotics additive分类
农业科技引用本文复制引用
刘银坤,李昊,李子昕,李知新,张玉彦,李文财,任建军,唐姝..牦牛源短小芽胞杆菌TS1的分离鉴定及其生物学特性研究[J].南京农业大学学报,2024,47(1):69-77,9.基金项目
宁夏自然科学基金项目(2022AAC02072) （2022AAC02072）
宁夏重点研发项目黄河生态流域生态环境保护与高质量发展科技支撑专项(21BEF02019) （21BEF02019）
