南京农业大学学报2024，Vol.47Issue(1)：78-86,9.DOI:10.7685/jnau.202211025
饲粮添加不同水平芦丁对肉鸡肉品质和抗氧化功能的影响
Effects of different levels of dietary rutin supplementation on meat quality and muscle antioxidant capacity of broilers
摘要
Abstract
[Objectives]This experiment was conducted to investigate the effects of different dietary levels of rutin on meat quality and muscle antioxidant capacity of broilers.[Methods]A total of 256 1-day-old healthy male broilers with similar body weight were randomly divided into 4 groups with 8 replicates per group and 8 broilers per replicate.The control group was fed basal diet,and the experimental groups were fed with the basal diet supplemented with 250,500 and 1 000 mg·kg-1 rutin,respectively.On the 42nd day of this experimental period,1 broiler(close to the average body weight)was selected from each replicate for slaughtering and sampling to determine the related indexes of muscle quality,moisture distribution,fatty acid content,antioxidant capacity in muscle.[Results]Compared with the control group,the supplementation of 250,500 and 1 000 mg·kg-1 rutin significantly reduced the drip loss of the pectoralis major(PM)muscle at postmortem 24 and 48 h(PT<0.05),and the supplementation of 250 and 500 mg·kg-1 rutin decreased the cooking loss and shear force values of the PM muscle,respectively(PT<0.05).The supplementation of 500 and 1 000 mg·kg-1 rutin significantly increased the pH24 h value of PM muscle(PT<0.05);the supplementation of 500 mg·kg-1 rutin significantly decreased the relaxation time T22 of free water in breast muscle of broilers(PT<0.05),and the pH24 h and drip loss at 24 and 48 h were linearly or quadratic correlated with the supplemental level of rutin(PT<0.05).In terms of fatty acid content,dietary 250 and 500 mg·kg-1 rutin significantly increased the content of stearic acid in PM muscle of broilers(PT<0.05),and dietary 500 and 1 000 mg·kg-1 rutin significantly increased the contents of arachidonic acid and polyunsaturated fatty acid(PUFA)in PM muscle of broilers(PT<0.05).At the same time,the contents of palmitoleic acid and tetracenoic acid in PM muscle of 500 mg·kg-1 rutin group significantly increased(PT<0.05),and the content of γ-linoleic acid of 1 000 mg·kg-1 rutin group significantly decreased(PT<0.05).In terms of antioxidant capacity,dietary 500 mg·kg-1 rutin significantly decreased MDA content in PM muscle(PT<0.05),increased T-SOD activity(PT<0.05),and significantly increased Nrf2 and SOD mRNA expressions levels in PM muscle(PT<0.05).250 mg·kg-1 rutin significantly increased SOD mRNA expression level(PT<0.05),and there was a linear or quadratic correlation between rutin supplemental level and MDA content,Nrf2 and GSH-Px mRNA expression level(P<0.05).[Conclusions]Dietary rutin improves meat quality of broilers by enhancing the water retention of muscle,increasing the pH24 h value of breast muscle and improving the proportion of fatty acids in breast muscle,which may be related to the activation of Nrf2 signaling pathway and the enhancement of antioxidant capacity.Under the conditions of this experiment,the appropriate supplementation of rutin is 500 mg·kg-1.关键词
芦丁/肉鸡/肌肉品质/脂肪酸/抗氧化Key words
rutin/broiler/muscle quality/fatty acid/antioxidant分类
农业科技引用本文复制引用
马龙飞,陈顺,周斌斌,刘慧娟,张佳琦,王恬,王超..饲粮添加不同水平芦丁对肉鸡肉品质和抗氧化功能的影响[J].南京农业大学学报,2024,47(1):78-86,9.基金项目
江苏省苏北科技计划项目(SZ-YC202146) （SZ-YC202146）
国家自然科学基金项目(31972598) （31972598）
