南京农业大学学报2024，Vol.47Issue(1)：183-192,10.DOI:10.7685/jnau.202211029
维甲酸对热应激小鼠睾丸损伤的保护作用
Protective effect of retinoic acid on testicular injury in heat stressed mice
摘要
Abstract
[Objectives]The aim of this experiment was to investigate the protective effect of retinoic acid on testicular injury in heat stressed mice.[Methods]Forty 7-week-old male ICR mice were randomly divided into four groups:control group(CON),heat stressed group(HTRA0),5 mg·kg-1·d-1retinoic acid-treated group(HTRA5)and 10 mg·kg-1·d-1retinoic acid-treated group(HTRA10).Mice were gavaged with appropriate doses of retinoic acid or corn oil and then subjected to systemic heat stress at 42℃for 2 h.The mice were slaughtered and sampled at the end of the test.[Results]Compared with the CON group,the testicular weight,minimum diameter,cross-sectional area,diameter,and epithelial height of spermatogenic tubules in the HTRA0 group were significantly reduced(P<0.05),the epididymal sperm motility significantly decreased(P<0.05),the malondialdehyde(MDA)content and the expression levels of antioxidant related genes(nrf2,ho-1,nqo1,sod1,gpx1)in testis tissue significantly increased(P<0.05),while total superoxide dismutase(T-SOD)activity significantly decreased(P<0.05),the mRNA expression level of bax significantly increased(P<0.05),the expression levels of blood-testis barrier related genes(occludin,zo-1,n-cadherin and connexin 43)significantly increased(P<0.05),and the expression levels of spermatogenesis related genes(plzf,c-kit)expression significantly increased(P<0.05).Compared with HTRA0,the cross-sectional area,diameter and epithelial height in the HTRA5 group significantly increased(P<0.05),the MDA content significantly decreased(P<0.05)while T-SOD activity significantly increased(P<0.05),and the mRNA expression levels of plzf and c-kit significantly decreased(P<0.05).Compared with HTRA0,the cross-sectional area,diameter and epithelial height of spermatogenic tubules in the HTRA10 group significantly increased(P<0.05),sperm motility significantly increased(P<0.05),MDA content significantly decreased(P<0.05),T-SOD activity significantly increased(P<0.05),and the mRNA expression levels of nqo1,bax,bcl-2,zo-1,n-cadherin,plzf and c-kit were significantly reduced(P<0.05).[Conclusions]Retinoic acid could improve testicular weight,testicular histomorphology,and epididymal sperm motility in heat-stressed mice.This protective effect may be achieved by increasing the antioxidant capacity of mouse testes,reducing germ cell apoptosis,enhancing the blood-testis barrier,and improving the spermatogenic microenvironment.关键词
热应激/维甲酸/小鼠/睾丸/精子活力/氧化应激/血睾屏障Key words
heat stress/retinoic acid/mouse/testicle/sperm motility/oxidative stress/blood testosterone barrier分类
农业科技引用本文复制引用
石一凡,李晓彤,锁云鹏,刘俊泽,李春梅,李延森..维甲酸对热应激小鼠睾丸损伤的保护作用[J].南京农业大学学报,2024,47(1):183-192,10.基金项目
中国博士后科学基金(2020M681648) （2020M681648）
江苏省博士后科研资助计划(2021K435C) （2021K435C）
江苏现代农业(生猪)产业技术体系建设项目(JATS[2022]083) （生猪）
