农业生物技术学报2024，Vol.32Issue(2)：247-258,12.DOI:10.3969/j.issn.1674-7968.2024.02.001
小麦与叶锈菌互作过程中TaGRF1-A基因功能的研究
Study on the Function of TaGRF1-A in the Interaction Between Wheat(Triticum aestivum)and Puccinia triticina
摘要
Abstract
The cloning of genes related to wheat(Triticum aestivum)resistance leaf rust and the in-depth study of their expression characteristics are of great significance for elucidating the molecular mechanisms of wheat resistance Puccinia triticina.By analyzing the RNA-seq transcriptome database obtained in the laboratory in the early stage,a gene belong to 14-3-3 family closely related to resistance to P.triticina infection was discovered,with significant differences in expression between compatible and incompatible combinations.In order to deeper understanding of the mechanism of this gene in wheat resistance to P.triticina infection,this study cloned the gene from wheat near isogenic lines TcLr26,the result of sequence alignment showed that the gene was TaGRF1-A in wheat.Preparation of polyclonal antibodies against TaGRF1-A protein,Western blot analysis showed that the antibody was able to bind specifically to TaGRF1-A protein in wheat,and showed an upregulation and then decrease expression trend induced by Puccinia triticina in the incompatible combinations.The virus induced gene silencing(VIGS)technology was used to reduce the expression of TaGRF1-A in incompatible combinations,inoculate with leaf rust showed a significant increase in the area of hypersensitive reaction(HR)and the number of haustorium mother cells(HMCs)at the site of P.triticina infection.After 15 d of inoculation with leaf rust,a spore of P.triticina appeared on the surface of the leaves,and the expression of pathogenesis-related(PR)genes were reduced.The results indicated that TaGRF1-A was positive regulating the wheat resistance to leaf rust infection.This study provides reference for further exploring the mechanism of TaGRF1-A in the interaction between wheat and leaf rust.关键词
小麦/叶锈菌/TaGRF1-A/多克隆抗体/病毒诱导的基因沉默(VIGS)Key words
Wheat/Puccinia triticina/TaGRF1-A/Polyclonal antibody/Virus-induced gene silencing(VIGS)分类
农业科技引用本文复制引用
顾佳,牛泽霖,王茜,王冬梅..小麦与叶锈菌互作过程中TaGRF1-A基因功能的研究[J].农业生物技术学报,2024,32(2):247-258,12.基金项目
国家自然科学基金(31171472 （）
31871548) （）
河北省研究生创新资助项目(CXZZBS2022246) （CXZZBS2022246）
河北省自然科学基金(C2023204054) （C2023204054）
