农业生物技术学报2024，Vol.32Issue(2)：283-298,16.DOI:10.3969/j.issn.1674-7968.2024.02.004
陆地棉磷脂酶C基因家族的鉴定及其在棉纤维发育过程中的表达分析
Identification of Gossypium hirsutum PLC Gene Family and Its Expression Analysis During Fiber Developmental Stages
摘要
Abstract
Phospholipase C(PLC)is an enzyme involved in the hydrolysis of phosphatidylinositol 4,5-diphosphate,and its downstream products are involved in the signal transduction of Ca2+ and protein kinase C.In this study,28 members of the GhPLC gene family were identified from the genome of Gossypium hirsutum'Nongdamian No.8'(NDM8)by using the Hidden Markov Model(PF09279 and PF04185).According to the phylogenetic analysis of protein sequences,GhPLC family members were divided into 2 subgroups:PIPLC(16)and nPLC(12).Gene duplication analysis showed that 50%of GhPIPLC and 92%of GhnPLC genes originated from segmental duplication and subjected to purification selection.The cis-acting elements analysis of the upstream 2000 bp sequence of GhPLC family genes as promoters found that all GhPLC gene promoters contained plant hormone or abiotic stress responsive cis-acting elements,indicating that GhPLCs could be induced by plant hormone or abiotic stresses.qPCR analysis showed that 15 GhPLC gene promoters contained gibberellin responsive-element,and 14 of them could be induced by gibberellin to up-regulate the expression.15 GhPLC gene promoters contained auxin-responsive elements,among which 14 GhPLC could be induced by auxin analogues to up-regulate the expression.25 of the 28 GhPLC gene family members were up-regulated in the initiation and elongation stages of cotton fiber developmental stages,accounting for 89%of the GhPLC gene family members.This study provides a reference for further study on the function of GhPLC gene in cotton fiber initiation and elongation stages.关键词
陆地棉/PLC基因家族/进化分析/启动子/表达分析Key words
Gossypum hirsutum/PLC gene family/Evolutionary analysis/Promoter/Expression analysis分类
农业科学引用本文复制引用
窦玲玲,牛换换,郑凯..陆地棉磷脂酶C基因家族的鉴定及其在棉纤维发育过程中的表达分析[J].农业生物技术学报,2024,32(2):283-298,16.基金项目
陕西省教育厅专项科研计划(22JK0602 （）
20JK0973) （）
陕西省大学生创新创业训练计划(S202310722092) （S202310722092）
安徽荃银高科种业股份有限公司资助(GMLM2023-09) （GMLM2023-09）
