农业生物技术学报2024，Vol.32Issue(2)：299-310,12.DOI:10.3969/j.issn.1674-7968.2024.02.005
茭白'浙农7号'不同生长阶段的叶片转录组学及激素水平分析
Analysis of Transcriptomics and Hormone Levels of Jiaobai'zhenong 7'in Different Growth Stages
摘要
Abstract
Jiaobai is formed when Ustilago esculenta infects and stimulates the Zizania latifolia plant,causing endogenous hormones changes that induce the host pregnancy and stimulate the stem of the plant to swell.So determination of dynamic changes of hormones and transcriptome is important to clarify the regulation mechanism of key genes in different stages of growth and development of Jiaobai.Illumina platform sequencing technology was used to sequence the transcripts of 4 growth stages(pre tillering stage,tillering stage,pre pregnancy stage and pregnancy stage)of Jiaobai varieties'zhenong 7'(ZN7)in this study.The results showed that total of 194 differentially expressed genes(DEGs)were screened in 4 growth stages,Gene Ontology(GO)function analysis showed that the DEGs in 4 growth stages of ZN7 were mainly involved in cell response to high light intensity and cell response to ultraviolet-A(UV-A)biological process.DEGs were mainly involved in photosynthesis,photosynthesis-antennal protein,carbon fixation in photosynthetic organisms etc.in KEGG enrichment analysis.Compared the contents of endogenous hormones in the 4 growth stages of ZN7,it was found that the trend of content changes of gibberellin(GA3),cytokinin(CTK),auxin(IAA)and abscisic acid(ABA)in each growth stage of Jiaobai were not consistent.The content of GA3 in pregnant period was significantly higher than that in tillering stage(P＜0.05),contents of IAA and ABA in each period were basically the same,which was late tillering stage＞early tillering stage＞pregnant stage,the CTK content reached the peak in the early tillering stage,which was significantly higher than that in the late tillering stage and pregnant stage(P＜0.05),but there was no significant difference among the other 3 stages.The results provide scientific basis for key genes screening and regulatory mechanisms illustrating at different growth stages of Jiaobai.关键词
茭白'浙农7号'/不同生长阶段/转录组/差异表达基因(DEGs)/内源激素Key words
Jiaobai'zhenong 7'/Different growth stages/Transcriptome/Differentially expressed genes(DEGs)/Endogenous hormone分类
农业科技引用本文复制引用
张珏锋,李芳,钟海英,陈建明..茭白'浙农7号'不同生长阶段的叶片转录组学及激素水平分析[J].农业生物技术学报,2024,32(2):299-310,12.基金项目
国家现代农业产业技术体系(CARS-24-G-07) （CARS-24-G-07）
浙江省"三农九方"科技协作计划项目(2022SNJF016 （）
2022SNJF082) （）
国家自然科学青年基金(32001963) （32001963）
