农业生物技术学报2024，Vol.32Issue(2)：322-333,12.DOI:10.3969/j.issn.1674-7968.2024.02.007
森林郁闭度和凋落物对延胡索生长和土壤酶化学计量的影响
Effects of Forest Canopy Density and Litter Manipulation on Corydalis yanhusuo Growth and Soil Enzyme Stoichiometry
摘要
Abstract
Canopy density is the main factor affecting herbaceous plant growth because it leads to insufficient light,thick litter layer,and gives allelopathy effect.The main objective of this study was to explore the canopy density(low,medium,and high)and litter effects on the growth of C.yanhusuo with the restriction of soil enzyme stoichiometry.Results revealed that low canopy density of Cunninghamia lanceolata significantly increased height(24%to 27%),SPAD(soil and plant analyzer development)value(11%to 33%),net photosynthetic rate(28%to 39%),stomatal conductance(28%to 35%)of C.yanhusuo.Also,low canopy density increased the intercellular carbon dioxide concentration(10%to 24%)in C.yanhusuo plants.Although,litter manipulation had no significant impacts on growth of C.yanhusuo.With low canopy density,litter retention significantly increased the content of soil-soluble organic nitrogen(N)(11%to 21%),and enzyme activity of β-1,4-glucosidase(BG),β-1,4-n-acetylglucosaminidase(NAG)and leucine aminopeptidase(LAP).Canopy density,litter manipulation and their interaction showed significant effects on values of SPAD,soil C,N,phosphorus(P)content and related enzymes.The interaction between canopy density and litter had significant effects on plant height and SPAD value(P＜0.01),which had a significant effect on the soil C,N and P content,and had a significant effect on the soil BG,NAG,LAP,and acid phosphatase(AP)activity(P＜0.01).Microbial P limitation was demonstrated among all treatments via BG/(LAP+NAG)and(LAP+NAG)/AP,which indicated that the soil lacked P for microorganisms availability.In litter retention treatment,the soil BG/AP ratio decreased with increasing canopy density,and suggested that reducing canopy density and retaining litter could alleviate the restriction of soil phosphorus.It was evident that there was significant positive correlation between soil BG/AP and plants growth parameters.The low canopy density with litter retention significantly improved the soil enzyme activities and phosphorus supply that increased the growth of C.yanhusuo.This study could possibly provide the theoretical basis to explore the habitat suitability of C.yanhusuo and other herbaceous wild plants endangered.关键词
凋落物/郁闭度/延胡索/土壤酶活性/酶化学计量/磷营养限制Key words
Litter manipulation/Canopy density/Corydalis yanhusuo/Soil enzyme activity/Enzyme stoichiometric/Phosphorus limitation分类
农业科技引用本文复制引用
张文卓,张前前,俞叶飞,迟晓立,何安国,王雨齐,吕强锋,李永春..森林郁闭度和凋落物对延胡索生长和土壤酶化学计量的影响[J].农业生物技术学报,2024,32(2):322-333,12.基金项目
国家自然科学基金(32071742) （32071742）
浙江省金华市科学技术研究计划重点项目(2021-2-004) （2021-2-004）
浙江省磐安县科技计划重点项目(磐科农202102 （）
磐科农202203) （）
