农业生物技术学报2024，Vol.32Issue(2)：344-354,11.DOI:10.3969/j.issn.1674-7968.2024.02.009
牦牛STAG3基因克隆及其在不同年龄段牦牛睾丸中的表达定位
Clone of STAG3 Gene and Its Expression and Localization in Yak(Bos grunniens)Testis with Different Ages
摘要
Abstract
Stromal antigen 3(STAG3),one component of adhesin complexes,plays important role in regulation of follicular development,premature ovarian failure and carcinogenesis in animals.However,its function and regulatory mechanisms are incompletely understood in yak(Bos grunniens).In the present study,the yak testicular tissues with different ages(2,4,6 and 8 years old)were collected.The coding sequence(CDS)of STAG3 gene was cloned using reverse transcription-PCR(RT-PCR).The interacted proteins and biological functions of STAG3 were predicted using STRING(functional protein association networks)and Gene Ontology(GO)procedures.Histomorphological structure of yak tissues was observed using hematoxylin-eosin(HE)staining.The localization and expression pattern analysis of STAG3 were evaluated using immunohistochemical(IHC)staining and qRT-PCR.The results showed that the CDS of yak STAG3 was 3679 bp with a 1659 bp open reading frame,and encoding 552 amino acids without transmembrane structure region.STAG3 might involve in the meiosis Ⅰ phase,synaptic re-combination and chromosome concentration.The STAG3 protein was widely expressed in testicular tissues,and were located mainly in primary spermatocyte and secondary spermatocyte.The highest expression level of STAG3 was presented in the yak testis of 2 years old,and was gradually decreased with ages.In conclusion,STAG3 was conservative in animal evolution,and widely expressed in the yak testises,which might play an important role in yak reproductive processes.This study provides basis for uncovering the function and mechanism of STAG3 in yak reproduction.关键词
牦牛/基质抗原3(STAG3)/基因克隆/生物信息学分析Key words
Yak/Stromal antigen 3(STAG3)/Gene cloning/Bioinformatics analysis分类
农业科技引用本文复制引用
石军,陈文丽,白旭,李建富,袁宝,赵兴绪,张全伟..牦牛STAG3基因克隆及其在不同年龄段牦牛睾丸中的表达定位[J].农业生物技术学报,2024,32(2):344-354,11.基金项目
国家自然科学基金(03120057) （03120057）
中国科学院"西部之光"(03321013) （03321013）
