农业生物技术学报2024，Vol.32Issue(2)：367-378,12.DOI:10.3969/j.issn.1674-7968.2024.02.011
运输应激对山羊胃壁组织细胞凋亡及凋亡相关基因Bcl-2和Bax表达的影响
Effects of Transport Stress on Apoptosis and Expression of Apoptosis-related Genes Bcl-2 and Bax in the Gastric Wall Tissues of Goat(Capra hircus)
摘要
Abstract
With the development of goat(Capra hircus)breeding,transportation-induced health problems in goats cannot be ignored.The aim of this study was to investigate the effects of transport stress on cell apoptosis and the expression of apoptosis-related genes,such as B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax)in gastric wall tissue of Ganxi goat.Twelve healthy one-year-old Ganxi male goats were randomly divided into control group(n=4),2 h transport group(n=4)and 6 h transport group(n=4).During the transportation process,food and water restrictions were carried out.After the transportation,tissues from stomach wall of the goats were collected and analyzed for relevant histology and quantification.Terminal dexynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL)results showed that the cell apoptotic rate of each stomach wall tissue in the 2 h transport group and the 6 h transport group was significantly increased(P＜0.05)compared to the control group.Additionally,the cell apoptotic rate of other gastric wall tissues,except for omasum,was obviously higher in the 6 h transport group than in the 2 h transport group(P＜0.05).Immunohistochemical results showed that Bax and Bcl-2 were mainly expressed in the mucosa epithelium of the anterior stomach(rumen,reticulum and omasum)and in the lamina propria of the abomasum.Bax expression in the 2 transport groups was clearly higher in the aforementioned areas of the stomach wall,with the decrease of Bcl-2 expression,but which was not obvious down-regulated in omasum.qPCR analysis revealed that the mRNA expression level of Bax was significantly up-regulated in other gastric wall tissues,except for omasum,in the 2 transport groups(P＜0.05),whereas the Bcl-2 mRNA expression was significantly down-regulated(P＜0.05).More importantly,Bax/Bcl-2 ratio in mRNA levels was significantly increased after transportation(P＜0.05).Western blot results also showed that there was a similar pattern in the Bax and Bcl-2 protein expression to that of mRNA.These findings indicated that transport stress could lead to cell apoptosis in goat stomach wall tissue through regulating the expression of Bax and Bcl-2.This work may be able to serve as a theoretical foundation for investigating strategies to reduce the damage of goat organs and tissues caused by transport stress.关键词
山羊/运输应激/胃壁组织/细胞凋亡/B细胞淋巴瘤-2(Bcl-2)/Bcl-2相关X蛋白(Bax)Key words
Goat/Transport stress/Gastric wall tissues/Apoptosis/B-cell lymphoma-2(Bcl-2)/Bcl-2 associated X protein(Bax)分类
农业科技引用本文复制引用
胡威,刘犇,郑文亚,方满新,胡迎东,章志涛,张雯苑,叶恬..运输应激对山羊胃壁组织细胞凋亡及凋亡相关基因Bcl-2和Bax表达的影响[J].农业生物技术学报,2024,32(2):367-378,12.基金项目
江西省教育厅科技项目(GJJ211630 （）
GJJ201622) （）
江西现代农业科研协同创新专项(JXXTCX201702) （JXXTCX201702）
