土地类芽胞杆菌NK3-4对藤仓镰孢菌基因表达的影响OACSTPCD

Paenibacillus terrae NK3-4 strain is found to be antagonistic to plant pathogens.However,the response mechanism of NK3-4 interaction with the pathogen remains unclear.In this study,the inhibitory effect of NK3-4 on rice(Oryza sativa)bakanae disease pathogenic Fusarium fujikuroi was measured,and a transcriptome sequencing analysis for the NK3-4 inhibited F.fujikuroi was taken.The results showed that NK3-4 inhibited the growth,pigment synthesis and conidia formation of F.fujikuroi,and damaged the mycelia.Among the 2291 differential expressed genes in F.fujikuri induced by NK3-4,the number of down-regulated genes was 2.44 times of up-regulated genes.And the down-regulated genes were mainly enriched in nucleus(GO:0005634),protein-containing complex(GO:0032991),intracellular non-membrane-bound organelles(GO:0043232)and other functional groups.Among the differential expressed genes,the number of down-regulated expressed genes related to pathogenesis(GO:0009405),toxin biosynthetic process(GO:0009403),aflatoxin biosynthetic process(GO:0045122),reproduction(GO:0000003)and other relative functions were more than that of the up-regulated genes.Meanwhile,of the differential expressed genes which involved in cell wall chitin biosynthetic process(GO:00060382),asexual reproduction(GO:0019954),conidia formation(GO:0048315),conidia development(GO:0061794),pigment biosynthetic process(GO:0046148)and other relative functions were all down-regulated expressed.The result of reverse transcription fluorescence quantitative PCR detection was consistent with transcriptome sequencing result(Pearson correlation index 0.90),indicating NK3-4 down-regulated the expression levels of genes in F.fujikuroi.Synthetic analysis showed that NK3-4 may inhibit F.fujikuroi by down-regulating the expression of genes,which related to growth and reproduction,pigment synthesis and spore production,and may reduce its virulence by inhibiting the biosynthesis of toxin in F.fujikuroi.The results provide a basis for further elucidating the molecular mechanism of NK3-4 interacting with F.fujikuroi.