日用化学工业（中英文）2024，Vol.54Issue(1)：57-64,8.DOI:10.3969/j.issn.2097-2806.2024.01.008
滨海刺芹愈伤组织提取物中4种多酚含量测定及抗氧化、抗炎活性研究
Determination of four polyphenols and study on antioxidant and anti-inflammatory activities of extracts from callus of Eryngium maritimum L.
肖雪 1张慧荣 1李硕雯 2李孙华 2郭苗苗 1李丽1
作者信息
- 1. 北京工商大学 轻工科学与工程学院 北京市植物资源开发重点实验室,北京 100048
- 2. 安赛搏(重庆)生物技术有限公司,重庆 400700
- 折叠
摘要
Abstract
The preparation of extracts from callus of Eryngium maritimum was conducted by hot water reflux method.A method for simultaneous determination of rosmarinic acid,cryptochlorogenic acid,hyperoside,quercetin-3-O-(6″-O-acetyl)-β-D-glucopyranoside in the callus extract of Eryngium maritimum was established based on high performance liquid chromatography.The scavenging ability of the extracts on DPPH and ABTS free radicals were determined.The screening samples were also assayed by the CCK-8 method to determine the concentration of drug administration and a model of HaCaT cell inflammation induced by Cutibacterium acnes was established to evaluate its anti-inflammatory efficacy.The results show that the extracts contain the highest amount of cryptochlorogenic acid at 5.23 mg/g,and the contents of rosmarinic acid,quercetin-3-O-(6″-O-acetyl)-β-D-glucopyranoside and hyperin were 1.30,0.50 and 0.08 mg/g,respectively.The extracts display antioxidant and anti-inflammatory abilities.The IC50 values of the extracts on DPPH and ABTS free radical scavenging ability are 0.323 9 and 0.296 5 mg/mL,respectively,and also have significant inhibitory effects on the secretion of IL-1β and IL-8 in HaCaT cells induced by Cutibacterium acnes(P<0.05).关键词
滨海刺芹/高效液相色谱/多酚/抗氧化/抗炎Key words
Eryngium maritimum L./high performance liquid chromatography/polyphenol/anti-oxidant/anti-inflammatory分类
化学化工引用本文复制引用
肖雪,张慧荣,李硕雯,李孙华,郭苗苗,李丽..滨海刺芹愈伤组织提取物中4种多酚含量测定及抗氧化、抗炎活性研究[J].日用化学工业（中英文）,2024,54(1):57-64,8.
