日用化学工业（中英文）2024，Vol.54Issue(1)：102-106,5.DOI:10.3969/j.issn.2097-2806.2024.01.014
高效液相色谱法测定化妆品中3种α-羟基酸及其酯
Determination of 3 kinds of α-hydroxy acids and their esters in cosmetics by high performance liquid chromatography
摘要
Abstract
A method for the simultaneous determination of 3 kinds of α-hydroxy acids and their esters in cosmetics,including gluconic acid,glucolactone and lactose acid,was developed by high performance liquid chromatography(HPLC),and the results were confirmed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The above three substances were extracted ultrasonically with water.The analytes were separated on a Comixsil HCS(4.6 mm×150 mm,3µm)column by elution with 0.04 mol/L diammonium hydrogen phosphate-acetonitrile as mobile phase,and detected at the wavelengths of 214 nm.Quantification analysis was performed by the external standard method.HPLC-MS/MS was used for gradient elution with 0.1%formic acid-acetonitrile containing 0.1%formic acid as mobile phase.The analytes were separated on a Waters T3-C18(2.1 mm×100 mm,1.8µm)column.Qualitative analysis was performed by electronic spray ionization under a negative ion mode with multiple reaction monitoring(MRM).The results indicate that gluconic acid and lactose acid show good linearity in its own concentration range with the correlation coefficient of R2>0.999.The detection limits of gluconic acid and glucolactone are 100 μg/g,and that of lactose acid is 500 μg/g.The average spiked recoveries of these three compounds in 4 cosmetic matrices are all in the range of 90.8%-110.1%,with the RSDs of 0.3%-4.4%.The results indicate that the method is simple,sensitive and accurate,and is suitable for the determination of 3 kinds of α-hydroxy acids and their esters in cosmetics.关键词
化妆品/葡糖酸/葡糖酸内酯/乳糖酸/高效液相色谱法/高效液相色谱-质谱联用法Key words
cosmetics/gluconic acid/glucolactone/lactose acid/HPLC/HPLC-MS/MS分类
化学化工引用本文复制引用
张丽媛,程巧鸳,陈岑,李泽桦,黄柳倩,戚绿叶..高效液相色谱法测定化妆品中3种α-羟基酸及其酯[J].日用化学工业（中英文）,2024,54(1):102-106,5.基金项目
2021年《化妆品安全技术规范》制修订项目 （）
2022年度浙江省药品监督管理局科技计划(2021036) （2021036）
